PXD009206 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Dose- and time-dependent effects of triethylene glycol dimethacrylate on the proteome of human THP-1 monocytes |
Description | Triethylene glycol dimethacrylate (TEGDMA) is commonly used in polymer resin-based dental materials. This projected investigated molecular mechanisms of TEGDMA toxicity by identifying time- and dose dependent effects on the proteome of human THP-1 monocytes. Effects of different concentrations (0.07mM-5mM) and exposure times (0-72h) of TEGDMA on cell viability, proliferation, and morphology were determined by a real-time viability assay, automated cell counting, and electron microscopy, and laid the fundament for choice of exposure scenarios in the proteomic experiments. Cells were metabolically labelled (SILAC), and exposed to 0.3mM or 2.5mM TEGDMA for 6h or 16h prior to LC-MS/MS analyses. Regulated proteins were analysed with the STRING database. Cells exposed to 0.3mM TEGDMA showed increased viability, and time-dependent upregulation of proteins associated with stress/oxidative stress, autophagy, and cytoprotective functions. Cells exposed to 2.5mM TEGDMA showed diminished viability, and a protein expression profile associated with oxidative stress, DNA-damage, mitochondrial dysfunction and cell-cycle inhibition. Altered expression of immune genes was observed in both groups. |
HostingRepository | PRIDE |
AnnounceDate | 2024-10-22 |
AnnouncementXML | Submission_2024-10-22_04:36:38.659.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Jack-Ansgar Bruun |
SpeciesList | scientific name: Homo sapiens (Human); NCBI TaxID: 9606; |
ModificationList | No PTMs are included in the dataset |
Instrument | Q Exactive |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2018-03-14 06:13:48 | ID requested | |
1 | 2018-07-31 07:18:09 | announced | |
⏵ 2 | 2024-10-22 04:36:39 | announced | 2024-10-22: Updated project metadata. |
Publication List
10.1111/eos.12559; |
Nilsen BW, Simon-Santamaria J, Ö, rtengren U, Jensen E, Bruun JA, Michelsen VB, S, ø, rensen KK, Dose- and time-dependent effects of triethylene glycol dimethacrylate on the proteome of human THP-1 monocytes. Eur J Oral Sci, 126(5):345-358(2018) [pubmed] |
Keyword List
curator keyword: Biological |
submitter keyword: THP1, SILAC, methacrylate |
Contact List
Ulf Örtengren |
contact affiliation | Department of Clinical Dentistry, UiT - The Arctic University of Norway, Tromsø, Norway |
contact email | ulf.ortengren@uit.no |
lab head | |
Jack-Ansgar Bruun |
contact affiliation | University of Tromsø |
contact email | jack-ansgar.bruun@uit.no |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD009206
- Label: PRIDE project
- Name: Dose- and time-dependent effects of triethylene glycol dimethacrylate on the proteome of human THP-1 monocytes