PXD009206

PXD009206 is an original dataset announced via ProteomeXchange.

Title	Dose- and time-dependent effects of triethylene glycol dimethacrylate on the proteome of human THP-1 monocytes
Description	Triethylene glycol dimethacrylate (TEGDMA) is commonly used in polymer resin-based dental materials. This projected investigated molecular mechanisms of TEGDMA toxicity by identifying time- and dose dependent effects on the proteome of human THP-1 monocytes. Effects of different concentrations (0.07mM-5mM) and exposure times (0-72h) of TEGDMA on cell viability, proliferation, and morphology were determined by a real-time viability assay, automated cell counting, and electron microscopy, and laid the fundament for choice of exposure scenarios in the proteomic experiments. Cells were metabolically labelled (SILAC), and exposed to 0.3mM or 2.5mM TEGDMA for 6h or 16h prior to LC-MS/MS analyses. Regulated proteins were analysed with the STRING database. Cells exposed to 0.3mM TEGDMA showed increased viability, and time-dependent upregulation of proteins associated with stress/oxidative stress, autophagy, and cytoprotective functions. Cells exposed to 2.5mM TEGDMA showed diminished viability, and a protein expression profile associated with oxidative stress, DNA-damage, mitochondrial dysfunction and cell-cycle inhibition. Altered expression of immune genes was observed in both groups.
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_04:36:38.659.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Jack-Ansgar Bruun
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	No PTMs are included in the dataset
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2018-03-14 06:13:48	ID requested
1	2018-07-31 07:18:09	announced
⏵ 2	2024-10-22 04:36:39	announced	2024-10-22: Updated project metadata.

10.1111/eos.12559;

Nilsen BW, Simon-Santamaria J, Ö, rtengren U, Jensen E, Bruun JA, Michelsen VB, S, ø, rensen KK, Dose- and time-dependent effects of triethylene glycol dimethacrylate on the proteome of human THP-1 monocytes. Eur J Oral Sci, 126(5):345-358(2018) [pubmed]

curator keyword: Biological

submitter keyword: THP1, SILAC, methacrylate

Ulf Örtengren
contact affiliation	Department of Clinical Dentistry, UiT - The Arctic University of Norway, Tromsø, Norway
contact email	ulf.ortengren@uit.no
lab head
Jack-Ansgar Bruun
contact affiliation	University of Tromsø
contact email	jack-ansgar.bruun@uit.no
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2018/07/PXD009206

PRIDE project URI

• PRIDE
  1. PXD009206
     1. Label: PRIDE project
     2. Name: Dose- and time-dependent effects of triethylene glycol dimethacrylate on the proteome of human THP-1 monocytes