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PXD009206

PXD009206 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleDose- and time-dependent effects of triethylene glycol dimethacrylate on the proteome of human THP-1 monocytes
DescriptionTriethylene glycol dimethacrylate (TEGDMA) is commonly used in polymer resin-based dental materials. This projected investigated molecular mechanisms of TEGDMA toxicity by identifying time- and dose dependent effects on the proteome of human THP-1 monocytes. Effects of different concentrations (0.07mM-5mM) and exposure times (0-72h) of TEGDMA on cell viability, proliferation, and morphology were determined by a real-time viability assay, automated cell counting, and electron microscopy, and laid the fundament for choice of exposure scenarios in the proteomic experiments. Cells were metabolically labelled (SILAC), and exposed to 0.3mM or 2.5mM TEGDMA for 6h or 16h prior to LC-MS/MS analyses. Regulated proteins were analysed with the STRING database. Cells exposed to 0.3mM TEGDMA showed increased viability, and time-dependent upregulation of proteins associated with stress/oxidative stress, autophagy, and cytoprotective functions. Cells exposed to 2.5mM TEGDMA showed diminished viability, and a protein expression profile associated with oxidative stress, DNA-damage, mitochondrial dysfunction and cell-cycle inhibition. Altered expression of immune genes was observed in both groups.
HostingRepositoryPRIDE
AnnounceDate2024-10-22
AnnouncementXMLSubmission_2024-10-22_04:36:38.659.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterJack-Ansgar Bruun
SpeciesList scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationListNo PTMs are included in the dataset
InstrumentQ Exactive
Dataset History
RevisionDatetimeStatusChangeLog Entry
02018-03-14 06:13:48ID requested
12018-07-31 07:18:09announced
22024-10-22 04:36:39announced2024-10-22: Updated project metadata.
Publication List
10.1111/eos.12559;
Nilsen BW, Simon-Santamaria J, Ö, rtengren U, Jensen E, Bruun JA, Michelsen VB, S, ø, rensen KK, Dose- and time-dependent effects of triethylene glycol dimethacrylate on the proteome of human THP-1 monocytes. Eur J Oral Sci, 126(5):345-358(2018) [pubmed]
Keyword List
curator keyword: Biological
submitter keyword: THP1, SILAC, methacrylate
Contact List
Ulf Örtengren
contact affiliationDepartment of Clinical Dentistry, UiT - The Arctic University of Norway, Tromsø, Norway
contact emailulf.ortengren@uit.no
lab head
Jack-Ansgar Bruun
contact affiliationUniversity of Tromsø
contact emailjack-ansgar.bruun@uit.no
dataset submitter
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Dataset FTP location
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