PXD009179

PXD009179 is an original dataset announced via ProteomeXchange.

Title	A proteomics of gills approach to understanding salinity adaptation of Scylla paramamosain
Description	Scylla paramamosain (Crustacea) is a commercially important euryhaline species distributed along the coast of southern China and other Indo-Pacific countries. However, a sudden variation in salinity will cause injury or even death of S. paramamosain. In this paper, we simulated a sudden decrease in salinity due to heavy precipitation in crab ponds. Comparison of gill microstructures of individuals in the control group and decreased salinity group showed gills became shorter and thicker, while the top of the filaments became swollen and then returned to normal after 120 h. A total of 3962 proteins were identified by proteomic sequencing of gills after 120 h under conditions of decreased salinity. 845 proteins were differentially expressed proteins: 371 up-regulated and 474 down-regulated. Of the enriched KEGG pathways, 20 were up-regulated and 14 were down-regulation (p<0.05). Among the significantly enriched up-regulated pathways, six were associated with amino acid metabolism and three were associated with Na+-K+-ATPase enzymatic activities. Pathways associated with redox metabolism and energy metabolism were identified. These results showed that in response to a decrease in salinity, S. paramamosain could adapt to the environment after 120 h. Molecular mechanism of this adaptation involved amino acid metabolism and Na+-K+-ATPase ion transport. Meanwhile, energy metabolism and redox metabolism were critical to the adaptation to a sudden decrease in salinity. This study, for the first time at the protein level, revealed the molecular mechanisms underlying salinity adaption of S. paramamosain and provides theoretical guidelines for the cultivation of S. paramamosain and other marine crustaceans.
HostingRepository	PRIDE
AnnounceDate	2018-07-31
AnnouncementXML	Submission_2018-07-31_07:28:37.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	wang huan
SpeciesList	scientific name: Scylla paramamosain; NCBI TaxID: 85552;
ModificationList	deamidated residue
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2018-03-12 02:28:52	ID requested
⏵ 1	2018-07-31 07:28:39	announced

Wang H, Wei H, Tang L, Lu J, Mu C, Wang C, A proteomics of gills approach to understanding salinity adaptation of Scylla paramamosain. Gene, 677():119-131(2018) [pubmed]

curator keyword: Biological

submitter keyword: Scylla paramamosain, Gill, iTRAQ protein profile, Sudden decrease in salinity, Adaptive mechanism

Chunlin Wang
contact affiliation	Key Laboratory of Applied Marine Biotechnology, Ministry of Education, Ningbo University, Ningbo 315211, Zhejiang, China
contact email	anghuan1@nub.edu.cn
lab head
wang huan
contact affiliation	honglingwei
contact email	632140981@qq.com
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD009179
     1. Label: PRIDE project
     2. Name: A proteomics of gills approach to understanding salinity adaptation of Scylla paramamosain