PXD009174

PXD009174 is an original dataset announced via ProteomeXchange.

Title	Integrated proteomic analysis of Tumor Necrosis Factor α and Interleukin 1 β-induced endothelial inflammation
Description	Objective The vascular endothelium provides a unique interaction plane for plasma proteins and leukocytes in inflammation. It has been established that particular proteins, including ICAM1, VCAM1 and SELE, are upregulated on the endothelial cell surface in the presence of pro-inflammatory cytokines Tumor Necrosis Factor α (TNFα) and Interleukin 1β (IL-1β). We now map cytokine-induced proteomic alterations to gain further insight into endothelial inflammation. Approach and results We evaluated alterations in the in-depth proteome, cell surface proteome and phosphoproteome after 24 hours of cytokine stimulation. In addition, protein expression profiles were measured after 4, 8, 16 and 24 hours of stimulation. We found that cytokine-stimulation induced a two-step response. After 4 hours, initial protein alterations were detected, including upregulation of ICAM1 and SELE, followed by a second burst of regulation after 8 to 16 hours, which included proteins involved in antigen processing, cytokine production, virus defense and ECM-interaction. Furthermore, we found that TNFα and IL-1β provoke a highly similar endothelial response with few specific alterations. Using a novel cell surface proteomics approach, we observed highly similar changes on the cell surface. Two surface proteins displayed altered labeling of specific epitopes, suggesting that these sites are modified or involved in interactions. Combined, our integrated proteomic data describes a full array of affected processes: leukocyte interaction, self-antigen presentation, cytokine production, nitric oxide production and extracellular matrix interaction. Conclusions Our study provides a detailed quantitative proteomic analysis of endothelial inflammation in which novel inflammation-responsive proteins were uncovered, and a two-step cytokine response was identified.
HostingRepository	PRIDE
AnnounceDate	2018-09-03
AnnouncementXML	Submission_2018-09-03_03:12:28.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Eelke Béguin
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	biotinylated residue; phosphorylated residue
Instrument	Orbitrap Fusion ETD

Revision	Datetime	Status	ChangeLog Entry
0	2018-03-12 01:59:57	ID requested
⏵ 1	2018-09-03 03:12:29	announced

B, é, guin EP, van den Eshof BL, Hoogendijk AJ, Nota B, Mertens K, Meijer AB, van den Biggelaar M, -induced endothelial inflammation. J Proteomics, 192():89-101(2019) [pubmed]

curator keyword: Biological, Biomedical

submitter keyword: Endothelium, inflammation, TNFα, IL-1β, proteomics, phosphoproteomics, cell surface proteomics, time-resolved

Maartje van den Biggelaar
contact affiliation	Sanquin Research, Department of Plasma Proteins, Amsterdam, The Netherlands
contact email	m.vandenbiggelaar@sanquin.nl
lab head
Eelke Béguin
contact affiliation	Sanquin Research
contact email	eelke.beguin@gmail.com
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD009174
     1. Label: PRIDE project
     2. Name: Integrated proteomic analysis of Tumor Necrosis Factor α and Interleukin 1 β-induced endothelial inflammation