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PXD009168

DataSet Summary

  • HostingRepository: MassIVE
  • AnnounceDate: 2018-03-12
  • AnnouncementXML: Submission_2018-03-12_07:46:07.xml
  • DigitalObjectIdentifier:
  • ReviewLevel: Non peer-reviewed dataset
  • DatasetOrigin: Original data
  • RepositorySupport: Supported dataset by repository
  • PrimarySubmitter: Mark Adamo
  • Title: Global assessment of Plk1 network dynamics identifies inhibition of PP6 as a mechanism to promote Aurora A activity
  • Description: Polo-like kinase 1 (Plk1) is an essential protein kinase that promotes faithful mitotic progression in eukaryotes. Plk1 subcellular localization and substrate interactions are tightly controlled and require binding of Plk1 to phosphorylated sequences. Here, we use quantitative proteomics to identify phosphorylation-dependent interactions within the Plk1 network in human mitotic HeLa cells using kinase inhibitors and a Plk1 mutant deficient in phosphorylation-dependent substrate binding. We find that many interactions are abolished upon kinase inhibition; however, a subset are protected from phosphatase opposition or are unopposed, resulting in persistent Plk1-substrate interactions. This subset includes Phosphoprotein Phosphatase 6 (PP6), whose activity towards Aurora kinase A (AURKA) is inhibited by Plk1. This Plk1-PP6 interaction creates a feedback loop that coordinates and reinforces the activities of Plk1 and AURKA during mitotic entry and is terminated by Plk1 degradation during mitotic exit. Collectively, this work provides a cellular mechanism for the observed regulation of AURKA by Plk1.
  • SpeciesList: scientific name: Homo sapiens; common name: human; NCBI TaxID: 9606;
  • ModificationList: Label:13C(6)15N(2); Label:13C(6)15N(4); L-methionine sulfoxide; O-phospho-L-serine; O-phospho-L-threonine; O4'-phospho-L-tyrosine; S-carboxamidomethyl-L-cysteine
  • Instrument: LTQ Orbitrap; instrument model: Q Exactive Plus

Dataset History

VersionDatetimeStatusChangeLog Entry
02018-03-09 13:58:54ID requested
12018-03-12 07:46:08announced

Publication List

  1. no publication

Keyword List

  1. submitter keyword: Polo-like kinase 1, Plk1, mitosis, phosphorylation, Phosphoprotein Phosphatase 6, Aurora kinase A, AURKA, PP6

Contact List

    Scott Gerber
    • contact affiliation: The Geisel School of Medicine at Dartmouth
    • contact email: scott.a.gerber@dartmouth.edu
    • lab head:
    Arminja Kettenbach
    • contact affiliation: The Geisel School of Medicine at Dartmouth
    • contact email: arminja.n.kettenbach@dartmouth.edu
    • lab head:
    Mark Adamo
    • contact affiliation: Dartmouth College
    • contact email: mark.e.adamo@dartmouth.edu
    • dataset submitter:

Full Dataset Link List

  1. MassIVE dataset URI
  2. Dataset FTP location
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