PXD009166

PXD009166 is an original dataset announced via ProteomeXchange.

Title	SUMO1-conjugation is altered during normal aging but not by increased amyloid burden
Description	SUMOylation is a post-translational protein modification that consists of the attachment of a SUMO (small ubiquitin-related modifier) moiety to a lysine residue of a target protein. Alteration in protein SUMOylation has been linked to the amyloid pathology apparent in Alzheimer’s disease. In the present work, we aimed to elucidate the role of protein SUMOylation during aging and increased amyloid burden in vivo using a His6-HA-SUMO1 knock-in mouse in the 5XFAD model of Alzheimer’s disease. We performed anti-HA based affinity purification from young (8 weeks) and old (36 weeks) mouse brains, followed by label-free quantification of purified proteins by LC-MS. Interestingly, we did not observe any alteration in the levels of SUMO1 conjugation related to Alzheimer’s disease, but found age-related alterations in global levels of SUMO1 conjugation. The identified SUMO1 candidate substrates are dominantly nuclear proteins, mainly involved in RNA processing. Our findings open novel directions of research for studying a functional link between SUMOylation and its role in guarding nuclear functions during aging.
HostingRepository	PRIDE
AnnounceDate	2018-04-20
AnnouncementXML	Submission_2018-04-20_05:25:21.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Olaf Jahn
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationList	sumoylated lysine
Instrument	Synapt MS

Revision	Datetime	Status	ChangeLog Entry
0	2018-03-09 09:43:49	ID requested
⏵ 1	2018-04-20 05:25:22	announced

Stankova T, Piepkorn L, Bayer TA, Jahn O, Tirard M, SUMO1-conjugation is altered during normal aging but not by increased amyloid burden. Aging Cell, 17(4):e12760(2018) [pubmed]

curator keyword: Biological, Biomedical

submitter keyword: Mouse, brain, affinity purification, post-translational modification, small ubiquitin-related modifier (SUMO), label-free quantification, aging, Alzheimer

Olaf Jahn
contact affiliation	Proteomics Group Max-Planck-Institute of Experimental Medicine Hermann-Rein-Str. 3 37075 Goettingen Germany
contact email	jahn@em.mpg.de
lab head
Olaf Jahn
contact affiliation	Proteomics Group, Max-Planck-Institute of Experimental Medicine, Göttingen
contact email	jahn@em.mpg.de
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD009166
     1. Label: PRIDE project
     2. Name: SUMO1-conjugation is altered during normal aging but not by increased amyloid burden