PXD009161 is an
original dataset announced via ProteomeXchange.
Dataset Summary
| Title | Nrf2 sequesters Keap1 preventing podosome disassembly |
| Description | Nrf2 is a transcription factor playing an essential role in stress response. A knowledge of the significance of Nrf2 in cell function has not, as yet, reached out beyond transactivation of gene expression. This paper shows that GDF-15 and SDF-1-induced angiogenesis strongly depends on Nrf2 presence, but is not related to its transcriptional activity. We propose, that Nrf2 serves as a protein restraining Keap1, its known transcriptional repressor. Deficiency of Nrf2 protein available for Keap1 leads to overabundance of RhoGAP1, the protein regulating Cdc42 activity, and impairs podosome assembly, thereby indisposing actin rearrangements, preventing migration and angiogenesis. These activities can be rescued by concomitant deletion of RhoGAP1 or Keap1. We suggest that a new Nrf2 function of a Keap1 scavenger implies revising the established murine model of Nrf2 deficiency as a transcriptional knock out (tKO) mouse. The N-terminal fragment of Nrf2, containing Neh2 domain binding Keap1, is present in these animals. Thus, regarding the function of Nrf2 as a protein sequestering Keap1, both published and unpublished data on Nrf2-Keap1 duet may gain new interpretation. |
| HostingRepository | PRIDE |
| AnnounceDate | 2024-10-22 |
| AnnouncementXML | Submission_2024-10-22_04:43:39.030.xml |
| DigitalObjectIdentifier | |
| ReviewLevel | Peer-reviewed dataset |
| DatasetOrigin | Original dataset |
| RepositorySupport | Unsupported dataset by repository |
| PrimarySubmitter | Dominik Cysewski |
| SpeciesList | scientific name: Homo sapiens (Human); NCBI TaxID: 9606; |
| ModificationList | No PTMs are included in the dataset |
| Instrument | Q Exactive |
Dataset History
| Revision | Datetime | Status | ChangeLog Entry |
|---|
| 0 | 2018-03-09 07:15:46 | ID requested | |
| 1 | 2018-11-28 04:38:39 | announced | |
| ⏵ 2 | 2024-10-22 04:43:47 | announced | 2024-10-22: Updated project metadata. |
Publication List
| Kloska D, Kopacz A, Cysewski D, Aepfelbacher M, Dulak J, Jozkowicz A, Grochot-Przeczek A, Nrf2 Sequesters Keap1 Preventing Podosome Disassembly: A Quintessential Duet Moonlights in Endothelium. Antioxid Redox Signal, 30(14):1709-1730(2019) [pubmed] |
| 10.1089/ars.2018.7505; |
Keyword List
| curator keyword: Biological |
| submitter keyword: cells,human |
Contact List
| Anna Grochot-Przeczek |
|---|
| contact affiliation | Department of Medical Biotechnology, Faculty of Biochemistry Biophysics and Biotechnology, Jagiellonian University, Gronostajowa 7, 30-387 Krakow, Poland |
| contact email | anna.grochot-przeczek@uj.edu.pl |
| lab head | |
| Dominik Cysewski |
|---|
| contact affiliation | Medical University of Bialystok |
| contact email | dominikcysewski@gmail.com |
| dataset submitter | |
Full Dataset Link List
Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2018/11/PXD009161 |
| PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD009161
- Label: PRIDE project
- Name: Nrf2 sequesters Keap1 preventing podosome disassembly
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