PXD009123 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | The secretome and N-glycosylation profiles of the charophycean green alga, Penium margaritaceum, resemble those of embryophytes |
Description | The secretome is defined as the population of proteins that are secreted into the extracellular environment. Many proteins that are secreted by eukaryotes are N-glycosylated; however, there are striking differences in the diversity and conservation of N-glycosylation patterns between taxa. For example, the secretome and N-glycosylation structures differ between land plants and chlorophyte green algae, but it is not clear when this divergence took place during plant evolution. A potentially valuable system to study this issue is provided by the charophycean green algae (CGA), the immediate ancestors of land plants. In this study, we used lectin affinity chromatography (LAC) coupled with mass spectrometry to characterize the secretome, including secreted N-glycoproteins, of Penium margaritaceum, a member of the CGA. The identified secreted proteins and N-glycans were compared to those known from the chlorophyte green alga Chlamydomonas reinhardtii and the model land plant, Arabidopsis thaliana, to establish their evolutionary context. Our approach allowed the identification of cell wall proteins and proteins modified with N-glycans that are identical to those of embryophytes, suggesting that the P. margaritaceum secretome is more closely related to those of land plants than to those of chlorophytes. The results of this study support the hypothesis that many of the proteins associated with plant cell wall modification, as well as other extracellular processes, evolved prior to the colonization of terrestrial habitats. |
HostingRepository | PRIDE |
AnnounceDate | 2018-03-26 |
AnnouncementXML | Submission_2018-03-26_00:31:11.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Eliel Ruiz-May |
SpeciesList | scientific name: Penium margaritaceum; NCBI TaxID: 102169; |
ModificationList | complex glycosylation |
Instrument | Q TRAP |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2018-03-06 08:49:32 | ID requested | |
⏵ 1 | 2018-03-26 00:31:12 | announced | |
Publication List
Ruiz-May E, S, ø, rensen I, Fei Z, Zhang S, Domozych DS, Rose JKC, The Secretome and N-Glycosylation Profiles of the Charophycean Green Alga, Penium margaritaceum, Resemble Those of Embryophytes. Proteomes, 6(2):(2018) [pubmed] |
Keyword List
curator keyword: Biological |
submitter keyword: secretome |
cell wall proteins |
embryophytes |
charophycean green algae |
N-glycosylation |
Contact List
Jocelyn K. C. Rose |
contact affiliation | Plant Biology Section, School of Integrative Plant Science, Cornell University |
contact email | jr286@cornell.edu |
lab head | |
Eliel Ruiz-May |
contact affiliation | Instituto de Ecología, A.C. |
contact email | eliel.ruiz@inecol.mx |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD009123
- Label: PRIDE project
- Name: The secretome and N-glycosylation profiles of the charophycean green alga, Penium margaritaceum, resemble those of embryophytes