PXD009114

PXD009114 is an original dataset announced via ProteomeXchange.

Title	Dystrophin isoform Dp71eΔ71 is involved in the neurite outgrowth and neuronal differentiation of PC12 cells
Description	The Dp71 protein is the most abundant dystrophin in the central nervous system (CNS). Several dystrophin Dp71 isoforms have been described and are classified into three groups, each with a different C- terminal end. However, the functions of Dp71 isoforms remain unknown. In the present study, we analyse the effect of Dp71eΔ71 overexpression on the neuronal differentiation of PC12 Tet-On cells. Overexpression of the dystrophin Dp71eΔ71 stimulates neuronal differentiation, increasing the percentage of cells with neurites and the neurite length. According to the 2-DE analysis, Dp71eΔ71 overexpression modified the protein expression profile of PC12 Tet-On cells that had been treated with neuronal growth factor (NGF) for nine days. Interestingly, all differentially expressed proteins were up-regulated compared to the control. The proteomic analysis showed that Dp71eΔ71 increases the expression of proteins with important roles in the differentiation process, such as the HspB1, S100A6, and K8 proteins involved in the cytoskeletal structure and the HCNP protein involved in neurotransmitter synthesis. The expression of the neuronal marker TH was also up- regulated.
HostingRepository	PRIDE
AnnounceDate	2018-05-28
AnnouncementXML	Submission_2018-05-28_00:30:18.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD009114
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Juan Pablo Reyes Grajeda
SpeciesList	scientific name: Rattus norvegicus (Rat); NCBI TaxID: 10116;
ModificationList	Deamidated; Methyl; Oxidation
Instrument	4800 Proteomics Analyzer

Revision	Datetime	Status	ChangeLog Entry
0	2018-03-05 05:21:09	ID requested
1	2018-04-09 01:34:40	announced
⏵ 2	2018-05-28 00:30:19	announced	Updated project metadata.

Garc, í, a-Cruz C, Merino-Jim, é, nez C, Ceja V, Arag, ó, n J, Siqueiros-M, á, rquez L, Reyes-Grajeda JP, Monta, ñ, ez C, is involved in neurite outgrowth and neuronal differentiation of PC12 cells. J Proteomics, 191():80-87(2019) [pubmed]

curator keyword: Biological

submitter keyword: Dystrophin

Dp71eΔ71

HspB1

neuronal differentiation

PC12 cells.

Juan Pablo Reyes Grajeda
contact affiliation	National Institute of Genomic Medicine
contact email	jreyes@inmegen.gob.mx
lab head
Juan Pablo Reyes Grajeda
contact affiliation	INMEGEN
contact email	jreyes@inmegen.gob.mx
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD009114
     1. Label: PRIDE project
     2. Name: Dystrophin isoform Dp71eΔ71 is involved in the neurite outgrowth and neuronal differentiation of PC12 cells