PXD009109

PXD009109 is an original dataset announced via ProteomeXchange.

Title	Oxidative posttranslational modifications of cysteine are associated to high intrinsic aerobic capacity in the skeletal and cardiac muscle
Description	Oxidative posttranslational modifications (Ox-PTMs) regulate cellular homeostasis in several tissues, including skeletal and cardiac muscles. The putative relationship between Ox-PTMs and intrinsic components of oxidative energy metabolism has not been previously described. We determined the metabolic phenotype and the Ox-PTM profile in the skeletal and cardiac muscles of rats selected for low (LCR) or high (HCR) intrinsic aerobic capacity. The HCR rats have a pronounced increase in mitochondrial content and antioxidant capacity when compared to LCR rats in the skeletal muscle, but only modest changes in the cardiac muscle. Redox proteomics analysis reveals that HCR and LCR rats have different Ox-PTM of cysteine (Cys) residue profile in the skeletal and cardiac muscles. HCR rats have higher number of oxidized Cys residues in the skeletal muscle and conversely display higher number of reduced Cys residues in the cardiac muscle than LCR rats. Most of the proteins with differentially oxidized Cys residues in the skeletal muscle are important regulators of the oxidative metabolism. The most significantly oxidized protein in the skeletal muscle of HCR rats is malate dehydrogenase (MDH1). Interestingly, HCR rats show higher MDH1 activity in the skeletal muscle, but not in the cardiac muscle. Thus, this study uncovers an association between Ox-PTMs and intrinsic aerobic capacity, providing new insights into the role of Ox-PTMs as essential signaling to maintain metabolic homeostasis in different muscle types.
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_04:43:32.467.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Animesh Sharma
SpeciesList	scientific name: Rattus norvegicus (Rat); NCBI TaxID: 10116;
ModificationList	Applied Biosystems cleavable ICAT(TM) heavy
Instrument	LTQ Orbitrap Elite

Revision	Datetime	Status	ChangeLog Entry
0	2018-03-05 04:28:48	ID requested
1	2019-11-12 10:27:54	announced
⏵ 2	2024-10-22 04:43:33	announced	2024-10-22: Updated project metadata.

10.1038/s41598-018-35728-2;

Souza RWA, Alves CRR, Medeiros A, Rolim N, Silva GJJ, Moreira JBN, Alves MN, Wohlwend M, Gebriel M, Hagen L, Sharma A, Koch LG, Britton SL, Slupphaug G, Wisl, ø, ff U, Brum PC, Differential regulation of cysteine oxidative post-translational modifications in high and low aerobic capacity. Sci Rep, 8(1):17772(2018) [pubmed]

curator keyword: Biological

submitter keyword: heart, Ox-PTM, MDH1, redox proteomics, exercise, ICAT, mitochondria, cysteine, cardiac, aerobic, skeleta,Oxidation, oxidative stress, posttranslational modifications, muscle

Geir Slupphaug
contact affiliation	Professor i molekylærbiologi og faglig leder av PROMEC Institutt for klinisk og molekylær medisin Fakultet for medisin og helsevitenskap geir.slupphaug@ntnu.no 72573076 91825455 Laboratoriesenteret, 231.05.034, Øya, Erling Skjalgssons g 1
contact email	geir.slupphaug@ntnu.no
lab head
Animesh Sharma
contact affiliation	Engineer at NTNU, Norway
contact email	sharma.animesh@gmail.com
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2019/11/PXD009109

PRIDE project URI

• PRIDE
  1. PXD009109
     1. Label: PRIDE project
     2. Name: Oxidative posttranslational modifications of cysteine are associated to high intrinsic aerobic capacity in the skeletal and cardiac muscle