⮝ Full datasets listing

PXD009095

PXD009095 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleMolecular basis of Tousled-Like-Kinase 2 (TLK2) activation – LC-MS/MS-based analysis of HEK293-expressed TLK2 phosphorylation state
DescriptionIn this study on human Tousled-Like-Kinase 2 (TLK2) mass spectrometry-based analysis was applied as part of a molecular characterization of TLK2 in the aim to increase the understanding of the mode of activation of this protein. Specifically, different TLK2 constructs were expressed analyzed to generate a “phosphorylation map” of the TLK2 protein. To analyse the phosphorylation state of TLK2 we used recombinant protein purified from HEK293. Samples of different TLK2 constructs were used for in-gel digestion and analysed by mass spectrometry. All samples were prepared and run in triplicates for label-free quantification. Explanation for MS raw files with TLK2 construct included: KD: Kinase-dead TLK2 construct with mutation D613A, N-terminal His- and Strep-tag. wt: Active form of TLK2, canonical sequence, N-terminal His- and Strep-tag. full: Full-length canonical sequence (1-772), N-terminal His- and Strep-tag. trunc: Truncated TLK2 sequence (191-772), N-terminal His- and Strep-tag.
HostingRepositoryPRIDE
AnnounceDate2024-10-22
AnnouncementXMLSubmission_2024-10-22_04:45:41.865.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterPatrick Ruether
SpeciesList scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationListphosphorylated residue
InstrumentQ Exactive
Dataset History
RevisionDatetimeStatusChangeLog Entry
02018-03-02 03:07:15ID requested
12018-09-04 08:11:03announced
22024-10-22 04:45:48announced2024-10-22: Updated project metadata.
Publication List
Mortuza GB, Hermida D, Pedersen AK, Segura-Bayona S, L, ó, pez-M, é, ndez B, Redondo P, R, ü, ther P, Pozdnyakova I, Garrote AM, Mu, ñ, oz IG, Villamor-Pay, à M, Jauset C, Olsen JV, Stracker TH, Montoya G, Molecular basis of Tousled-Like Kinase 2 activation. Nat Commun, 9(1):2535(2018) [pubmed]
10.1038/s41467-018-04941-y;
Keyword List
curator keyword: Biological
submitter keyword: Human, TLK2, HEK293, Phosphosites
Contact List
Jesper Velgaard Olsen
contact affiliationNNF Center for Protein Research, University of Copenhagen
contact emailjesper.olsen@cpr.ku.dk
lab head
Patrick Ruether
contact affiliationNNF Center for Protein Research - University of Copenhagen
contact emailpatrick.ruether@cpr.ku.dk
dataset submitter
Full Dataset Link List
Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2018/09/PXD009095
PRIDE project URI
Repository Record List
[ + ]