PXD009088

PXD009088 is an original dataset announced via ProteomeXchange.

Title	SWATH-LC/MS/MS of the proteomic response in Campylobacter jejuni 81-176 to sublethal concentrations of seven different bile acids
Description	To characterize the adaptation processes of C. jejuni to sublethal concentrations of bile acids, that are a crucial component of the intestinal antimicrobial defense, including cholic- , chenodeoxycholic-, taurocholic-, glycocholic-, deoxycholic-, lithocholic-, and ursodeoxycholic acid, we used proteome profiling by label-free mass spectrometry (SWATH-MS). The most toxic bile acids, deoxycholic- and chenodeoxycholic acid induced the most significant changes in protein expression. In detail we found a significant down-regulation of in principle all basic biosynthetic pathways and a general decrease of the transcription machinery. Concurrently, an induction of factors involved in detoxification of reactive oxygen species, protein folding, and bile acid exporting efflux pumps was detected. The metabolism was optimized in such a way that the more energy-efficient aerobic respiration pathway was increased, while the energy-inefficient anaerobic branches of the electron transport chain were down-regulated. Uptake of amino acids in order to catabolize them was also increased. These results show that C. jejuni has a well-built, differentiated system of adaptation to bile acids stress. The findings of this study will enhance the understanding of the biology on the interaction of C. jejuni with the human intestine, and may provide clues to future medical treatment and prevention of C. jejuni infections.
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_04:43:31.459.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Christof Lenz
SpeciesList	scientific name: Campylobacter jejuni subsp. jejuni 81-176; NCBI TaxID: 354242;
ModificationList	No PTMs are included in the dataset
Instrument	TripleTOF 5600

Revision	Datetime	Status	ChangeLog Entry
0	2018-03-02 01:01:01	ID requested
1	2018-10-01 02:57:31	announced
⏵ 2	2024-10-22 04:43:38	announced	2024-10-22: Updated project metadata.

Masanta WO, Zautner AE, Lugert R, Bohne W, Gross U, Leha A, Dakna M, Lenz C, Proteome Profiling by Label-Free Mass Spectrometry Reveals Differentiated Response of Campylobacter jejuni 81-176 to Sublethal Concentrations of Bile Acids. Proteomics Clin Appl, 13(3):e1800083(2019) [pubmed]

10.1002/prca.201800083;

curator keyword: Biomedical

submitter keyword: SWATH, bile acids,Campylobacter

Christof Lenz
contact affiliation	Core Facility Proteomics Institute of Clinical Chemistry University Medical Center Göttingen
contact email	christof.lenz@mpibpc.mpg.de
lab head
Christof Lenz
contact affiliation	Max Planck Institute for Biophysical Chemistry
contact email	christof.lenz@mpibpc.mpg.de
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2018/10/PXD009088

PRIDE project URI

• PRIDE
  1. PXD009088
     1. Label: PRIDE project
     2. Name: SWATH-LC/MS/MS of the proteomic response in Campylobacter jejuni 81-176 to sublethal concentrations of seven different bile acids