PXD009084

PXD009084 is an original dataset announced via ProteomeXchange.

Title	Identification and characterization of a ribosome receptor for starvation-induced ribophagy
Description	The lysosome degrades and recycles macromolecules, signals to the master growth regulator mTORC1, and is associated with human disease. Here, we performed quantitative proteomic analyses of lysosomes rapidly isolated using the LysoIP method and find that nutrient levels and mTOR dynamically modulate the lysosomal proteome. We focus on NUFIP1, a protein that upon mTORC1 inhibition redistributes from the nucleus to autophagosomes and lysosomes. Upon these conditions, NUFIP1 interacts with ribosomes and delivers them to autophagosomes by directly binding to LC3B. The starvation-induced degradation of ribosomes via autophagy (ribophagy) depends on the capacity of NUFIP1 to bind LC3B and promotes cell survival. We conclude that NUFIP1 is a receptor for the selective autophagy of ribosomes.
HostingRepository	PRIDE
AnnounceDate	2018-06-21
AnnouncementXML	Submission_2018-06-21_06:36:24.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Ivonne Heinze
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Orbitrap Fusion Lumos

Revision	Datetime	Status	ChangeLog Entry
0	2018-03-01 03:22:04	ID requested
⏵ 1	2018-06-21 06:36:25	announced

Wyant GA, Abu-Remaileh M, Frenkel EM, Laqtom NN, Dharamdasani V, Lewis CA, Chan SH, Heinze I, Ori A, Sabatini DM, NUFIP1 is a ribosome receptor for starvation-induced ribophagy. Science, 360(6390):751-758(2018) [pubmed]

curator keyword: Biological

submitter keyword: lysosome, mTOR, ribophagy

Alessandro Ori
contact affiliation	Leibniz Institute on Aging – Fritz Lipmann Institute (FLI
contact email	alessandro.ori@leibniz-fli.de
lab head
Ivonne Heinze
contact affiliation	Leibniz Institute on Aging
contact email	ivonne.heinze@leibniz-fli.de
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2018/06/PXD009084

PRIDE project URI

• PRIDE
  1. PXD009084
     1. Label: PRIDE project
     2. Name: Identification and characterization of a ribosome receptor for starvation-induced ribophagy