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PXD009083

PXD009083 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleQuantitative tandem affinity purification, an effective tool to investigate protein complex composition in plant hormone signaling: strigolactones in the spotlight
DescriptionPhytohormones tightly regulate plant growth by integrating changing environmental and developmental cues. Although the key players have been identified in many plant hormonal pathways, the molecular mechanisms and mode of action of perception and signaling remain incompletely resolved. Characterization of protein partners of known signaling components provides insight into the formed protein complexes, but, unless quantification is involved, does not deliver much, if any, information about the dynamics of the induced or disrupted protein complexes. Therefore, in proteomics research, the discovery of what actually triggers, regulates or interrupts the composition of protein complexes is gaining importance. Here, tandem affinity purification coupled to mass spectrometry (TAP-MS) is combined with label-free quantification (LFQ) to a highly valuable tool to detect physiologically relevant, dynamic protein-protein interactions in Arabidopsis thaliana cell cultures. To demonstrate its potential, we focus on the signaling pathway of one of the most recently discovered phytohormones, strigolactones.
HostingRepositoryPRIDE
AnnounceDate2024-10-22
AnnouncementXMLSubmission_2024-10-22_04:43:08.362.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterSylwia Struk
SpeciesList scientific name: Arabidopsis thaliana (Mouse-ear cress); NCBI TaxID: 3702;
ModificationListmonohydroxylated residue; acetylated residue; iodoacetamide derivatized residue
InstrumentLTQ Orbitrap Velos
Dataset History
RevisionDatetimeStatusChangeLog Entry
02018-03-01 03:19:00ID requested
12018-04-26 03:51:13announced
22018-05-15 08:20:46announcedUpdated publication reference for PubMed record(s): 29755490.
32024-10-22 04:43:16announced2024-10-22: Updated project metadata.
Publication List
10.3389/fpls.2018.00528;
Struk S, Braem L, Walton A, De Keyser A, Boyer FD, Persiau G, De Jaeger G, Gevaert K, Goormachtig S, Quantitative Tandem Affinity Purification, an Effective Tool to Investigate Protein Complex Composition in Plant Hormone Signaling: Strigolactones in the Spotlight. Front Plant Sci, 9():528(2018) [pubmed]
Keyword List
curator keyword: Biological
submitter keyword: qTAP, plant hormone signaling,strigolactones, SMXL7
Contact List
Sofie Goormachtig
contact affiliationDepartment of Plant Biotechnology and Bioinformatics, Ghent University, 9052 Ghent, Belgium Center for Plant Systems Biology, VIB, 9052 Ghent, Belgium
contact emailsogoo@psb.vib-ugent.be
lab head
Sylwia Struk
contact affiliationGent University
contact emailsystr@psb.vib-ugent.be
dataset submitter
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Dataset FTP location
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PRIDE project URI
Repository Record List
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