PXD009083

PXD009083 is an original dataset announced via ProteomeXchange.

Title	Quantitative tandem affinity purification, an effective tool to investigate protein complex composition in plant hormone signaling: strigolactones in the spotlight
Description	Phytohormones tightly regulate plant growth by integrating changing environmental and developmental cues. Although the key players have been identified in many plant hormonal pathways, the molecular mechanisms and mode of action of perception and signaling remain incompletely resolved. Characterization of protein partners of known signaling components provides insight into the formed protein complexes, but, unless quantification is involved, does not deliver much, if any, information about the dynamics of the induced or disrupted protein complexes. Therefore, in proteomics research, the discovery of what actually triggers, regulates or interrupts the composition of protein complexes is gaining importance. Here, tandem affinity purification coupled to mass spectrometry (TAP-MS) is combined with label-free quantification (LFQ) to a highly valuable tool to detect physiologically relevant, dynamic protein-protein interactions in Arabidopsis thaliana cell cultures. To demonstrate its potential, we focus on the signaling pathway of one of the most recently discovered phytohormones, strigolactones.
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_04:43:08.362.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Sylwia Struk
SpeciesList	scientific name: Arabidopsis thaliana (Mouse-ear cress); NCBI TaxID: 3702;
ModificationList	monohydroxylated residue; acetylated residue; iodoacetamide derivatized residue
Instrument	LTQ Orbitrap Velos

Revision	Datetime	Status	ChangeLog Entry
0	2018-03-01 03:19:00	ID requested
1	2018-04-26 03:51:13	announced
2	2018-05-15 08:20:46	announced	Updated publication reference for PubMed record(s): 29755490.
⏵ 3	2024-10-22 04:43:16	announced	2024-10-22: Updated project metadata.

10.3389/fpls.2018.00528;

Struk S, Braem L, Walton A, De Keyser A, Boyer FD, Persiau G, De Jaeger G, Gevaert K, Goormachtig S, Quantitative Tandem Affinity Purification, an Effective Tool to Investigate Protein Complex Composition in Plant Hormone Signaling: Strigolactones in the Spotlight. Front Plant Sci, 9():528(2018) [pubmed]

curator keyword: Biological

submitter keyword: qTAP, plant hormone signaling,strigolactones, SMXL7

Sofie Goormachtig
contact affiliation	Department of Plant Biotechnology and Bioinformatics, Ghent University, 9052 Ghent, Belgium Center for Plant Systems Biology, VIB, 9052 Ghent, Belgium
contact email	sogoo@psb.vib-ugent.be
lab head
Sylwia Struk
contact affiliation	Gent University
contact email	systr@psb.vib-ugent.be
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2018/04/PXD009083

PRIDE project URI

• PRIDE
  1. PXD009083
     1. Label: PRIDE project
     2. Name: Quantitative tandem affinity purification, an effective tool to investigate protein complex composition in plant hormone signaling: strigolactones in the spotlight