PXD009078

PXD009078 is an original dataset announced via ProteomeXchange.

Title	Identification of ADAM12S-interacting proteins in HeLa cells
Description	ADAM12 is an A Disintegrin And Metalloproteinases (ADAMs). It is composed of signaling sequence, prodomain, metalloproteinase, disintegrin, cysteine-rich, EGF-like, transmembrane domains, and a cytoplasmic tail. ADAM12 is highly expressed in multiple cancers and is a diagnostic and prognostic biomarker for the proliferation, migration, and invasion of cancer cells. ADAM12 has several isoforms resulted from splicing variants with different subcellular localization and biological functions. Two most important isoforms are the prototype membrane-anchored long isoform ADAM12L and the secreted short isoform ADAM12S. Both of them contain the N-terminal 704 amino acids while their C-termini are different with each other. ADAM12S has extra 34 amino acids at its C-terminus, which replace the 171-amino acid transmembrane and cytoplasmic tail in ADAM12L. In this work, we use immunoprecipitation and quantitative proteomics to identify the interacting proteins of the short form of ADAM12 (ADAM12S).
HostingRepository	PRIDE
AnnounceDate	2022-02-28
AnnouncementXML	Submission_2022-02-28_02:31:06.721.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	lipeng xiong
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	iodoacetamide derivatized residue
Instrument	LTQ Orbitrap Elite

Revision	Datetime	Status	ChangeLog Entry
0	2018-03-01 01:16:55	ID requested
⏵ 1	2022-02-28 02:31:07	announced

Xiong L, Yan W, Zubia E, Zhou Y, Zhang Y, Duan Q, Narayan M, Xu G, Quantitative proteomics and biochemical analyses reveal the role of endoplasmin in the regulation of the expression and secretion of A Disintegrin And Metalloproteinase 12. J Proteomics, 182():34-44(2018) [pubmed]

curator keyword: Biological

submitter keyword: HeLa,ADAM12S,interacting proteins,LC-MS/MS,label free quantification

Guoqiang Xu
contact affiliation	College of Pharmaceutical Sciences Soochow University Yunxuan Building 1328 199 Ren'ai Road, Suzhou, Jiangsu 215123, China
contact email	gux2002@suda.edu.cn
lab head
lipeng xiong
contact affiliation	soochow university
contact email	xionglipeng1218@qq.com
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD009078
     1. Label: PRIDE project
     2. Name: Identification of ADAM12S-interacting proteins in HeLa cells