PXD009073

PXD009073 is an original dataset announced via ProteomeXchange.

Title	Label-free quantitative analysis of PKA-dependent proteome changes in Trypanosoma brucei
Description	Protein kinase A (PKA), the main effector of second messenger cAMP, is highly conserved among eukaryotes and a paradigm for the mechanisms of regulation of protein kinases by ligands. The unique PKA holoenzymes in the phylogenetically distant protozoan parasite Trypanosoma are unresponsive to cAMP in vitro and in vivo. By small molecule screening and optimization, we designed direct, membrane-permeable activators binding with a kD of 9 nM to the CNB pockets of the T. brucei regulatory PKA subunit. 7-Cyano-7-deazainosine has low toxicity and thus is a perfect tool to explore cAMP-independent PKA signaling in these important pathogens. This project describes the PKA-inducible proteome of the bloodstream stage of T. brucei.
HostingRepository	PRIDE
AnnounceDate	2019-02-25
AnnouncementXML	Submission_2019-04-03_04:37:15.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Dupuy Jean-William
SpeciesList	scientific name: Trypanosoma brucei; NCBI TaxID: 5691;
ModificationList	deaminated residue; monohydroxylated residue; acetylated residue; iodoacetamide derivatized residue
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2018-02-28 07:47:49	ID requested
1	2019-02-25 06:38:32	announced
⏵ 2	2019-04-03 04:37:16	announced	Updated publication reference for PubMed record(s): 30926779.

Bachmaier S, Volpato Santos Y, Kramer S, Githure GB, Kl, ö, ckner T, Pepperl J, Baums C, Schenk R, Schwede F, Genieser HG, Dupuy JW, Forn, é I, Imhof A, Basquin J, Lorentzen E, Boshart M, Nucleoside analogue activators of cyclic AMP-independent protein kinase A of Trypanosoma. Nat Commun, 10(1):1421(2019) [pubmed]

curator keyword: Biological

submitter keyword: Protein kinase A, PKA, Trypanosoma, 7-Cyano-7-deazainosine, label-free quantitative proteome analysis

Michael Boshart
contact affiliation	Biocenter, Faculty of Biology, Genetics, Ludwig-Maximilians-University Munich (LMU), 82152 Martinsried, Germany
contact email	boshart@lmu.de
lab head
Dupuy Jean-William
contact affiliation	Plateforme Proteome Bordeaux
contact email	jean-william.dupuy@u-bordeaux.fr
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2019/02/PXD009073

PRIDE project URI

• PRIDE
  1. PXD009073
     1. Label: PRIDE project
     2. Name: Label-free quantitative analysis of PKA-dependent proteome changes in Trypanosoma brucei