PXD009071

PXD009071 is an original dataset announced via ProteomeXchange.

Title	Proteomic analysis of S-nitrosylated nuclear proteins in rat cortical neurons
Description	S-nitrosothiol Resin Assisted Capture(SNORAC) coupled with mass spectrometry analysis has been used to study S-nitrosylated proteins in rat cortical neurons. Proteins were captured on the resin by covalently attaching their former S-NO moiety. Then, nistrosylated proteins were eluted by on-bead digestion. Formerly S-nitrosylated peptides were also eluted from the beads in a second step, and analyzed separately for assessment of the nitrosylation site (qualitative analysis). Out of over 600 nitrosyltated proteins, 131 proteins have never been shown to be S-nitrosylated in any system and 612 are new targets of S-nitrosylation in cortical neurons. The site(s) of Snitrosylation were also identified for 59% of the targets.
HostingRepository	PRIDE
AnnounceDate	2018-07-11
AnnouncementXML	Submission_2018-07-11_05:55:37.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Marco Gaspari
SpeciesList	scientific name: Rattus norvegicus (Rat); NCBI TaxID: 10116;
ModificationList	methylthiolated residue; (18)O label at both C-terminal oxygens; monohydroxylated residue; N6: N6-dimethyl-L-lysine; iodoacetamide derivatized residue
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2018-02-28 07:21:22	ID requested
⏵ 1	2018-07-11 05:55:38	announced

Smith JG, Aldous SG, Andreassi C, Cuda G, Gaspari M, Riccio A, Proteomic analysis of S-nitrosylated nuclear proteins in rat cortical neurons. Sci Signal, 11(537):(2018) [pubmed]

curator keyword: Biological

submitter keyword: S-nitrosylation, cortical neurons, rat, q-exactive

Marco Gaspari
contact affiliation	Dept Clinical and Experimental Medicine, University of Catanzaro, Viale Europa, 88100 Catanzaro (Italy)
contact email	gaspari@unicz.it
lab head
Marco Gaspari
contact affiliation	University of Catanzaro
contact email	gaspari@unicz.it
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2018/07/PXD009071

PRIDE project URI

• PRIDE
  1. PXD009071
     1. Label: PRIDE project
     2. Name: Proteomic analysis of S-nitrosylated nuclear proteins in rat cortical neurons