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DataSet Summary

  • HostingRepository: PRIDE
  • AnnounceDate: 2018-06-12
  • AnnouncementXML: Submission_2018-07-11_08:21:18.xml
  • DigitalObjectIdentifier:
  • ReviewLevel: Peer-reviewed dataset
  • DatasetOrigin: Original data
  • RepositorySupport: Unsupported dataset by repository
  • PrimarySubmitter: Francesca Romana Dani
  • Title: Honeybee antennal proteome is shaped by caste and task more than by age
  • Description: Olfaction and chemical communication are fundamental for coordination in insect societies, especially in species forming large colonies. Reproductive and task partitioning suggest that colony members belonging to different castes or subcastes or performing different tasks during their life (polyethism) may produce different semiochemicals and be differently sensitive to the variety of pheromones and/or environmental odours. The main peripheral olfactory organs are the antennal chemosensilla, where the early olfactory processes take place. At this stage, members of two different families of soluble chemosensory proteins (Odorant Binding Proteins -OBPs-, Chemosensory Proteins -CSPs-) show a remarkable affinity for different odorants and act as carriers while a further family, the Niemann Pick type C2 proteins (NPC2) may have a similar function, although this has not been fully demonstrated. Sensillar lymph also contains Odorant degrading enzymes (ODEs) which are involved in inactivation through degradation of the chemical signals, once the message is conveyed. Despite their importance in chemical communication, poor is known about how proteins involved in peripheral olfaction and, more generally antennal proteins, differ in honeybees of different caste, task and age. Here we investigate for the first time, using a shotgun proteomic approach, the antennal profile of honeybee of different castes (queens and workers) and of workers performing different tasks (nursers, guards and foragers) by controlling for the potential confounding effect of age. Regarding olfactory proteins, major differences were observed between queens and workers, some of which were found to be more abundant in queens (OBP3, OBP18, NPC2-1) and others to be more abundant in workers (OBP15, OBP21, CSP1, CSP3); while between workers performing different tasks, OBP14 was more abundant in nurses with respect to guards and foragers, and two ODEs were more abundant in guards with respect to workers of 2nd week. Apart from proteins involved in olfaction, we have found that the antennal proteomes are mainly characterized by castes and tasks, while age has a limited effect on antennal protein profile.
  • SpeciesList: scientific name: Apis mellifera (Honeybee); NCBI TaxID: 7460;
  • ModificationList: monohydroxylated residue; iodoacetamide derivatized residue
  • Instrument: LTQ Orbitrap

Dataset History

VersionDatetimeStatusChangeLog Entry
02018-02-28 02:49:06ID requested
12018-06-11 23:51:46announced
22018-07-11 08:21:20announcedUpdated publication reference for PubMed record(s): 29973886.

Publication List

  1. Iovinella I, Cappa F, Cini A, Petrocelli I, Cervo R, Turillazzi S, Dani FR, Antennal Protein Profile in Honeybees: Caste and Task Matter More Than Age. Front Physiol, 9():748(2018) [pubmed]

Keyword List

  1. curator keyword: Biological
  2. submitter keyword: LC-MS/MS, nurse, guards, foragers, queen, Apis mellifera

Contact List

    Francesca Romana Dani
    • contact affiliation: Department of Biology, UniversitÓ degli studi di Firenze
    • contact email: francescaromana.dani@unifi.it
    • lab head:
    Francesca Romana Dani
    • contact affiliation: University of Firenze
    • contact email: francescaromana.dani@unifi.it
    • dataset submitter:

Full Dataset Link List

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