PXD009061

PXD009061 is an original dataset announced via ProteomeXchange.

Title	Comparison of proteomic sample preparation and data analysis methods by means of human follicular fluids
Description	In-depth proteome exploration of complex body fluids is a challenging task that requires optimal sample preparation and analysis in order to reach novel and meaningful insights. Analysis of follicular fluids is similarly difficult as that of blood serum due to the ubiquitous presence of several highly abundant proteins and a wide range of protein concentrations. Therefore, the accessibility of this complex body fluid for liquid chromatography-tandem mass spectrometry (LC/MS/MS) analysis is a challenging opportunity to gain insights into the physiological status or to identify new diagnostic and prognostic markers for e.g. the treatment of infertility. We compared different sample preparation methods (FASP, eFASP and in-solution digestion) and three different data analysis software packages (Proteome Discoverer with SEQUEST and Mascot, Maxquant with Andromeda) in conjunction with semi- and full-tryptic databank search approaches in order to obtain a maximum coverage of the proteome.
HostingRepository	PRIDE
AnnounceDate	2018-12-04
AnnouncementXML	Submission_2018-12-04_00:15:06.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Roland Lehmann
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	monohydroxylated residue; iodoacetamide derivatized residue; deamidated residue
Instrument	Orbitrap Fusion ETD

Revision	Datetime	Status	ChangeLog Entry
0	2018-02-28 00:33:39	ID requested
⏵ 1	2018-12-04 00:15:07	announced

Lehmann R, Schmidt A, Pastuschek J, M, ü, ller MM, Fritzsche A, Dieterle S, Greb RR, Markert UR, Slevogt H, Comparison of sample preparation techniques and data analysis for the LC-MS/MS-based identification of proteins in human follicular fluid. Am J Reprod Immunol, 80(2):e12994(2018) [pubmed]

curator keyword: Technical

submitter keyword: Proteomics, Follicular fluids, Sample preparation, data analysis

Prof. Hortense Slevogt
contact affiliation	Host Septomics Research Centre Jena University Hospital
contact email	hortense.slevogt@med.uni-jena.de
lab head
Roland Lehmann
contact affiliation	University Hospital Jena Septomics
contact email	roland.lehmann@med.uni-jena.de
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2018/12/PXD009061

PRIDE project URI

• PRIDE
  1. PXD009061
     1. Label: PRIDE project
     2. Name: Comparison of proteomic sample preparation and data analysis methods by means of human follicular fluids