PXD009037

PXD009037 is an original dataset announced via ProteomeXchange.

Title	Proteomic characterization of Zamilon vitis virions produced in various contexts
Description	Acanthamoeba-infecting Mimiviridae belong to three clades: Mimiviruses (A), Moumouviruses (B) and Megaviruses (C). The uniquely complex mobilome of these giant viruses includes virophages and linear 7 kb-DNA molecules called “transpovirons”. We recently isolated a new virophage (Zamilon vitis) and two new transpovirons (maBtv and mvCtv) respectively associated to B-clade and C-clade Mimiviridae. We used the capacity of the Zamilon virophage to replicate both on B-clade and C-clade host viruses to investigate the three partite interaction network governing the propagation of transpovirons. We notably performed proteomic comparisons of host viruses and virophage particles carrying or cleared of transpovirons in search of proteins involved in this adaptation process. These experiments revealed that transpoviron-encoded proteins are synthetized during the combined Mimiviridae/virophage/transpoviron replication process and are specifically incorporated into the virophage and Mimiviridae virions together with the cognate transpoviron DNA. This is a unique example of intricate commensalism in the viral world, where the Zamilon virophage and the transpoviron depend on their host giant virus to replicate, and the transpoviron depends on the virophage to propagate from one host virus to another.
HostingRepository	PRIDE
AnnounceDate	2020-04-14
AnnouncementXML	Submission_2020-04-14_02:08:03.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Yohann Couté
SpeciesList	scientific name: unclassified virophages; NCBI TaxID: 552364; scientific name: Mimiviridae; NCBI TaxID: 549779;
ModificationList	monohydroxylated residue; acetylated residue; iodoacetamide derivatized residue
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2018-02-26 03:46:43	ID requested
⏵ 1	2020-04-14 02:08:07	announced

Jeudy S, Bertaux L, Alempic JM, Lartigue A, Legendre M, Belmudes L, Santini S, Philippe N, Beucher L, Biondi EG, Juul S, Turner DJ, Cout, é Y, Claverie JM, Abergel C, Exploration of the propagation of transpovirons within Mimiviridae reveals a unique example of commensalism in the viral world. ISME J, 14(3):727-739(2020) [pubmed]

submitter keyword: Mimiviridae, Giant virus, virophage, transpoviron, virion proteomics

Virginie Brun
contact affiliation	EDyP
contact email	virginie.brun@cea.fr
lab head
Yohann Couté
contact affiliation	EDyP
contact email	yohann.coute@cea.fr
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD009037
     1. Label: PRIDE project
     2. Name: Proteomic characterization of Zamilon vitis virions produced in various contexts