PXD009035

PXD009035 is an original dataset announced via ProteomeXchange.

Title	Structural analysis of human ARS2, a platform for co-transcriptional RNA sorting
Description	ARS2 is a highly conserved metazoan protein involved in numerous aspects of nuclear RNA metabolism. As a direct partner of the nuclear cap-binding complex (CBC) it mediates interactions with diverse RNA processing and transport machineries in a transcript-dependent manner. Here we present the human ARS2 crystal structure, which exhibits similarities and metazoan-specific differences to the plant homologue SERRATE, most notably an additional RRM domain. We present biochemical, biophysical and cellular interactome data comparing wild type and mutant ARS2 that identify regions critical for interactions with FLASH (involved in histone mRNA biogenesis), NCBP3 (a putative cap-binding protein involved in mRNA export) and single-stranded RNA. We show that FLASH and NCBP3 have overlapping binding sites on ARS2 and that CBC-ARS2-NCBP3 form a ternary complex that is mutually exclusive with CBC-ARS-PHAX (involved in snRNA export). Our results support that mutually exclusive higher order CBC-ARS2 complexes are critical in determining Pol II transcript fate.
HostingRepository	PRIDE
AnnounceDate	2018-09-03
AnnouncementXML	Submission_2019-02-12_08:41:27.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Frank Stein
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	No PTMs are included in the dataset
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2018-02-26 03:22:59	ID requested
1	2018-09-03 03:45:55	announced
⏵ 2	2019-02-12 08:41:29	announced	Updated publication reference for PubMed record(s): 29703953.

Schulze WM, Stein F, Rettel M, Nanao M, Cusack S, a platform for co-transcriptional RNA sorting. Nat Commun, 9(1):1701(2018) [pubmed]

curator keyword: Biological

submitter keyword: ARS2, protein interaction

Stephen Cusack
contact affiliation	European Molecular Biology Laboratory, Grenoble Cedex 9, France. Tel: +(33)476207238 Email: cusack@embl.fr
contact email	cusack@embl.fr
lab head
Frank Stein
contact affiliation	EMBL
contact email	frank.stein@embl.de
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2018/09/PXD009035

PRIDE project URI

• PRIDE
  1. PXD009035
     1. Label: PRIDE project
     2. Name: Structural analysis of human ARS2, a platform for co-transcriptional RNA sorting