PXD009028

PXD009028 is an original dataset announced via ProteomeXchange.

Title	A Chemical Biology Toolbox to Study Protein Methyltransferases and Epigenetic Signaling
Description	Protein methyltransferases (PMTs) comprise a major class of epigenetic regulatory enzymes with therapeutic relevance. Here we present a collection of chemical probes and associated reagents and data to elucidate the function of human and murine PMTs in cellular studies. Our collection provides inhibitors and antagonists that together modulate most of the key regulatory methylation marks on histones H3 and H4, providing an important resource for modulating cellular epigenomes. We describe a comprehensive and comparative characterization of the probe collection with respect to their potency, selectivity, and mode of inhibition. We demonstrate the utility of this collection in CD4+ T cell differentiation assays revealing the remarkable potential of individual probes to alter multiple T cell subpopulations with important implications for T cell-mediated processes such as inflammation and immuno-oncology. In particular, we demonstrate a role for DOT1L in limiting Th1 cell differentiation and maintaining lineage integrity.
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_04:43:11.888.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Jenny Ward
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	iodoacetamide derivatized residue
Instrument	Q Exactive HF; Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2018-02-23 07:39:45	ID requested
1	2018-12-19 04:30:55	announced
2	2019-02-15 09:04:00	announced	Updated publication reference for PubMed record(s): 30604761.
⏵ 3	2024-10-22 04:43:16	announced	2024-10-22: Updated project metadata.

10.1038/s41467-018-07905-4;

Scheer S, Ackloo S, Medina TS, Schapira M, Li F, Ward JA, Lewis AM, Northrop JP, Richardson PL, Kaniskan H Ü, Shen Y, Liu J, Smil D, McLeod D, Zepeda-Velazquez CA, Luo M, Jin J, Barsyte-Lovejoy D, Huber KVM, De Carvalho DD, Vedadi M, Zaph C, Brown PJ, Arrowsmith CH, A chemical biology toolbox to study protein methyltransferases and epigenetic signaling. Nat Commun, 10(1):19(2019) [pubmed]

curator keyword: Technical

submitter keyword: LFQ,Epigenetics, Chemical probes

Kilian Huber
contact affiliation	Structural Genomics Consortium (SGC), Chemical biology Group, University of Oxford, United Kingdom. also affiliated with: Target Discovery Institute, Nuffield Department of Medicine, University of Oxford, United Kingdom.
contact email	kilian.huber@sgc.ox.ac.uk
lab head
Jenny Ward
contact affiliation	University of Oxford
contact email	jennifer.ward@ndm.ox.ac.uk
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2018/12/PXD009028

PRIDE project URI

• PRIDE
  1. PXD009028
     1. Label: PRIDE project
     2. Name: A Chemical Biology Toolbox to Study Protein Methyltransferases and Epigenetic Signaling