PXD009026

PXD009026 is an original dataset announced via ProteomeXchange.

Title	DNA mismatch repair activity of MutLalpha is regulated by CK2-dependent phosphorylation of MLH1
Description	Mass spectrometry based PTM phosphorylation analysis to study regulation mechanism of MUTL alpha, a heterodimer consisting of MLH1 and PMS2 and a key player in DNA mismatch repair (MMR). It could be demonstrated that phosphorylation of MLH1 by Casein Kinase II (CK2) at amino acid position 477 can switch off MMR activity in vitro.
HostingRepository	PRIDE
AnnounceDate	2018-08-29
AnnouncementXML	Submission_2018-08-28_23:32:11.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Sebastian Funke
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	phosphorylated residue; monohydroxylated residue; iodoacetamide derivatized residue
Instrument	LTQ Orbitrap

Revision	Datetime	Status	ChangeLog Entry
0	2018-02-23 05:48:57	ID requested
⏵ 1	2018-08-28 23:32:13	announced

We, ß, becher IM, Hinrichsen I, Funke S, Oellerich T, Plotz G, Zeuzem S, Grus FH, Biondi RM, Brieger A, is regulated by CK2-dependent phosphorylation of MLH1 (S477). Mol Carcinog, 57(12):1723-1734(2018) [pubmed]

ProteomeXchange project tag: Human Proteome Project

curator keyword: Biological

submitter keyword: MUTL alpha, HEK293T cells, immunoprecipitation, phosphorylation, LC-MS/MS

Franz H. Grus
contact affiliation	Experimental Ophthalmology Ophthalmology University Medical Center Mainz Germany
contact email	grus@eye-research.org
lab head
Sebastian Funke
contact affiliation	Experimental Ophthalmology, Ophthalmology, University Medical Center Mainz
contact email	sfunke@eye-research.org
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2018/08/PXD009026

PRIDE project URI

• PRIDE
  1. PXD009026
     1. Label: PRIDE project
     2. Name: DNA mismatch repair activity of MutLalpha is regulated by CK2-dependent phosphorylation of MLH1