PXD009024

PXD009024 is an original dataset announced via ProteomeXchange.

Title	NanoLC-MSMS TMT comparison of circulated and unstimulated in vitro cultured reticulocytes
Description	Triplicate samples of unstimulated in vitro culture-derived reticulocytes (derived from CD34+ cells) and cultured reticulocytes circulated overnight in an ex vivo circulation system were analysed through TMT-based quantitative proteomics to explore the impact of shear stress on reticulocyte protein abundance.
HostingRepository	PRIDE
AnnounceDate	2018-08-03
AnnouncementXML	Submission_2018-08-06_04:18:58.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Pedro Moura
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	No PTMs are included in the dataset
Instrument	Orbitrap Fusion

Revision	Datetime	Status	ChangeLog Entry
0	2018-02-23 05:31:12	ID requested
1	2018-08-03 09:00:34	announced
⏵ 2	2018-08-06 04:18:59	announced	Updated publication reference for PubMed record(s): 30076174.

Moura PL, Hawley BR, Mankelow TJ, Griffiths RE, Dobbe JGG, Streekstra GJ, Anstee DJ, Satchwell TJ, Toye AM, Non-muscle myosin II drives vesicle loss during human reticulocyte maturation. Haematologica, 103(12):1997-2007(2018) [pubmed]

curator keyword: Biological

submitter keyword: Human, nanoLC-MSMS, reticulocyte, erythrocyte, erythropoiesis

Ashley Mark Toye
contact affiliation	Department of Biochemistry, Biomedical Sciences Building, University of Bristol, Bristol, United Kingdom
contact email	ash.m.toye@bristol.ac.uk
lab head
Pedro Moura
contact affiliation	Department of Biochemistry, Biomedical Sciences Building, University of Bristol, Bristol, United Kingdom
contact email	p.moura@bristol.ac.uk
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2018/08/PXD009024

PRIDE project URI

• PRIDE
  1. PXD009024
     1. Label: PRIDE project
     2. Name: NanoLC-MSMS TMT comparison of circulated and unstimulated in vitro cultured reticulocytes