PXD009016

PXD009016 is an original dataset announced via ProteomeXchange.

Title	MPK4 phosphorylation dynamics and interacting proteins in plant immunity
Description	The Arabidopsis MAP kinase 4 (MPK4) has been proposed as negative player in plant immunity, but it is activated by pathogen-associated molecular patterns (PAMPs), such as flg22. The molecular mechanisms by which MPK4 is activated and controls plant defense remain elusive. In this study, we investigated the Arabidopsis defense against a bacterial pathogen Pseudomonas syringae pv. tomato (Pst) DC3000 when the MPK4 is overexpressed. We showed an increase in pathogen resistance and suppression of jasmonic acid (JA) signaling in the MPK4 overexpressing (OE) plants. We also showed that the OE plants are very sensitive to flg22-triggered reactive oxygen species (ROS) burst in guard cells, which resulted in an enhanced stomatal closure, compared to wild-type (WT). During the flg22 activation, dynamic phosphorylation of MPK4 within and outside of the conserved TEY activation loop was observed. To elucidate how MPK4 functions during the defense response, we used immunoprecipitation coupled with mass spectrometry (IP-MS) to identify MPK4 interacting proteins in the absence and presence of flg22. Quantitative proteomic analysis revealed a shift in MPK4-associated protein network, providing insight into the molecular functions of MPK4 at a systems level.
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_04:43:23.295.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Tong Zhang
SpeciesList	scientific name: Arabidopsis thaliana (Mouse-ear cress); NCBI TaxID: 3702;
ModificationList	acetylated residue; iodoacetamide derivatized residue
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2018-02-23 01:17:42	ID requested
1	2019-01-14 03:05:22	announced
⏵ 2	2024-10-22 04:43:31	announced	2024-10-22: Updated project metadata.

Zhang T, Schneider JD, Lin C, Geng S, Ma T, Lawrence SR, Dufresne CP, Harmon AC, Chen S, MPK4 Phosphorylation Dynamics and Interacting Proteins in Plant Immunity. J Proteome Res, 18(3):826-840(2019) [pubmed]

10.1021/acs.jproteome.8b00345;

curator keyword: Biological

submitter keyword: Arabidopsis MPK4

innate immunity

protein-protein interaction

flg22

kinase substrate

IP-MS

Sixue Chen
contact affiliation	University of Florida
contact email	schen@ufl.edu
lab head
Tong Zhang
contact affiliation	Pacific Northwest National Lab
contact email	tongzhanguf@gmail.com
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2019/01/PXD009016

PRIDE project URI

• PRIDE
  1. PXD009016
     1. Label: PRIDE project
     2. Name: MPK4 phosphorylation dynamics and interacting proteins in plant immunity