PXD009012

PXD009012 is an original dataset announced via ProteomeXchange.

Title	ARIH2 is a novel Vif-dependent regulator of CUL5-mediated APOBEC3G degradation in HIV infection
Description	Discovery of novel host-virus interactions leads to a better understanding of mechanisms underlying infection and points to potential therapeutic targets at the interface between virus and host proteins. Recently, global, virus-host interaction networks have been mapped using affinity purification-mass spectrometry (AP-MS) approaches, but these studies do not provide information about dynamic remodeling of host complexes during infection. Here, we describe a novel quantitative proteomics approach in the context of HIV infection to unravel dynamics of the Cullin RING E3 ligase 5 (CRL5) complex, which is hijacked by HIV Vif to degrade the viral restriction factor of the APOBEC3 family. Generating a dynamic and quantitative interaction network of CRL5 under various infection conditions, we identify the E3 ligase ARIH2 as novel regulator of APOBEC3G degradation, which is essential for HIV infectivity in primary CD4+ T-cells. ARIH2 acts in a “tag-team” mechanism that accelerates ubiquitin chain formation on APOBEC3G through CUL5Vif/CBFß by priming the substrate with mono-ubiquitination. Finally, our data suggest a general role for ARIH2 in CRL5 substrate ubiquitination in host cells.
HostingRepository	PRIDE
AnnounceDate	2019-04-11
AnnouncementXML	Submission_2019-07-03_00:20:31.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Ruth Huttenhain
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Orbitrap Fusion

Revision	Datetime	Status	ChangeLog Entry
0	2018-02-22 03:00:55	ID requested
1	2019-04-11 00:59:00	announced
⏵ 2	2019-07-03 00:20:32	announced	Updated publication reference for PubMed record(s): 31253590.

H, ü, ttenhain R, Xu J, Burton LA, Gordon DE, Hultquist JF, Johnson JR, Satkamp L, Hiatt J, Rhee DY, Baek K, Crosby DC, Frankel AD, Marson A, Harper JW, Alpi AF, Schulman BA, Gross JD, Krogan NJ, ARIH2 Is a Vif-Dependent Regulator of CUL5-Mediated APOBEC3G Degradation in HIV Infection. Cell Host Microbe, 26(1):86-99.e7(2019) [pubmed]

curator keyword: Biomedical

submitter keyword: host-virus interactions, AP-MS, ARIH2, CUL5, Cullin RING E3 ligases, HIV

Nevan J. Krogan
contact affiliation	University of California San Francisco
contact email	Nevan.Krogan@ucsf.edu
lab head
Ruth Huttenhain
contact affiliation	UCSF
contact email	ruth.huttenhain@ucsf.edu
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD009012
     1. Label: PRIDE project
     2. Name: ARIH2 is a novel Vif-dependent regulator of CUL5-mediated APOBEC3G degradation in HIV infection