PXD009003

PXD009003 is an original dataset announced via ProteomeXchange.

Title	Exploring bioactive peptides from bacterial secretomes using PepSAVI-MS: identification and characterization of Bac-21 from Enterococcus faecalis pPD1
Description	Background. As current methods for antibiotic drug discovery are being outpaced by the rise of antimicrobial resistance, new methods and innovative technologies are necessary to replenish our dwindling arsenal of antimicrobial agents. To this end, we developed the PepSAVI-MS pipeline to expedite the search for natural product bioactive peptides. Results. Herein we demonstrate expansion of PepSAVI-MS for the discovery of bacterial-sourced bioactive peptides through identification of the bacteriocin Bac-21 from Enterococcus faecalis pPD1. Minor pipeline modifications including implementation of bacteria-infused agar diffusion assays and optional digestion of peptide libraries highlight the versatility and wide adaptability of the PepSAVI-MS pipeline. Additionally, we have experimentally validated the primary protein sequence of the active, mature Bac-21 peptide for the first time and have confirmed identity with respect to primary sequence and post-translational processing. Conclusions: Successful application of PepSAVI-MS to bacterial secretomes as demonstrated herein establishes proof-of-principle for use in novel microbial bioactive peptide discovery.
HostingRepository	PRIDE
AnnounceDate	2018-07-23
AnnouncementXML	Submission_2018-07-23_07:14:25.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD009003
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Christine Kirkpatrick
SpeciesList	scientific name: Enterococcus faecalis (Streptococcus faecalis); NCBI TaxID: 1351;
ModificationList	Oxidation; Acetyl; Carbamidomethyl
Instrument	TripleTOF 5600; LTQ Orbitrap

Revision	Datetime	Status	ChangeLog Entry
0	2018-02-21 02:54:17	ID requested
⏵ 1	2018-07-23 07:14:26	announced

Kirkpatrick CL, Parsley NC, Bartges TE, Wing CE, Kommineni S, Kristich CJ, Salzman NH, Patrie SM, Hicks LM, Exploring bioactive peptides from bacterial secretomes using PepSAVI-MS: identification and characterization of Bac-21 from Enterococcus faecalis pPD1. Microb Biotechnol, 11(5):943-951(2018) [pubmed]

curator keyword: Biological

submitter keyword: Bioactive peptides, antimicrobial peptides (AMPs), bacterial secretome, Enterococcus faecalis, peptidomics, mass spectrometry, natural products

Leslie M. Hicks
contact affiliation	UNC Chapel Hill
contact email	lmhicks@unc.edu
lab head
Christine Kirkpatrick
contact affiliation	UNC Chapel Hill
contact email	ckirkpat@live.unc.edu
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2018/07/PXD009003

PRIDE project URI

• PRIDE
  1. PXD009003
     1. Label: PRIDE project
     2. Name: Exploring bioactive peptides from bacterial secretomes using PepSAVI-MS: identification and characterization of Bac-21 from Enterococcus faecalis pPD1