PXD008999

PXD008999 is an original dataset announced via ProteomeXchange.

Title	Proteomic analysis of human sclera tissue using high resolution mass spectrometry
Description	The sclera is white, tough outer covering, known for providing structural support to the eyeball and the intraocular contents. Any abnormality in sclera results in disorders such as conjunctivitis, episcleritis, scleritis and hence a proper investigation will help in preventing the same. In the current study, we carried out an in-depth proteomic analysis of human sclera in which the proteins were extracted from the sclera tissue and subjected to both in-gel and in-solution digestion using trypsin as the proteolytic enzyme. The peptides were analyzed using Orbitrap Fusion Tribrid mass spectrometerand the data acquired was searched against Human RefSeq 75 protein database using SEQUEST HT and Mascot which resulted in the identification of 4,493 non-redundant proteins.The outcome of this study will facilitate in discovery of potential candidate biomarkers aiding in curbing the scleral disorders.
HostingRepository	PRIDE
AnnounceDate	2019-02-21
AnnouncementXML	Submission_2019-02-21_08:02:18.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Keshava Prasad T. S.
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	monohydroxylated residue; acetylated residue; iodoacetamide derivatized residue
Instrument	Orbitrap Fusion Lumos

Revision	Datetime	Status	ChangeLog Entry
0	2018-02-21 02:29:31	ID requested
⏵ 1	2019-02-21 08:02:19	announced

Mohanty V, Subbannayya Y, Najar MA, Pinto SM, Kasaragod S, Karuppiah H, Sreeramulu B, Singh KK, Dalal S, Manikkoth S, Arunachalam C, Prasad TSK, Murthy KR, Proteomics and Visual Health Research: Proteome of the Human Sclera Using High-Resolution Mass Spectrometry. OMICS, 23(2):98-110(2019) [pubmed]

curator keyword: Biological

submitter keyword: Eye proteome, omics technology, protein abundance, tandem mass spectrometry, protein profiling

T. S. Keshava Prasad
contact affiliation	Center for Systems Biology and Molecular Medicine, Yenepoya(Deemed to be University), Mangalore 575018
contact email	keshav@ibioinformatics.org
lab head
Keshava Prasad T. S.
contact affiliation	Institute of Bioinformatics, Bangalore, Karnataka, India.
contact email	keshav@ibioinformatics.org
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2019/02/PXD008999

PRIDE project URI

• PRIDE
  1. PXD008999
     1. Label: PRIDE project
     2. Name: Proteomic analysis of human sclera tissue using high resolution mass spectrometry