PXD008993

PXD008993 is an original dataset announced via ProteomeXchange.

Title	Acrylamide acute neurotoxicity in adult zebrafish
Description	Acute exposure to acrylamide (ACR), a type-2 alkene, may lead to a ataxia, skeletal muscles weakness and numbness of the extremities in exposed human and laboratory animals. Recently, a zebrafish model for ACR neurotoxicity mimicking most of the pathophysiological processes described in mammalian models, was generated in 8 days post-fertilization larvae. In order to better understand the predictive value of the zebrafish larvae model of acute ACR neurotoxicity, in the present manuscript the ACR acute neurotoxicity has been characterized in the brain of adult zebrafish, and the results compared with those obtained with the whole-larvae. Although qualitative and quantitative analysis of the data shows important differences in the ACR effects between the adult brain and the whole-larvae, the overall effects of ACR in adult zebrafish, including a significant decrease in locomotor activity, altered expression of transcriptional markers of proteins involved in synaptic vesicle cycle, presence of ACR-adducts on cysteine residues of some synaptic proteins, and changes in the profile of some neurotransmitter systems, are similar to those described in the larvae. Thus, these results support the suitability of the zebrafish ACR acute neurotoxicity recently developed in larvae for screening of molecules with therapeutic value to treat this toxic neuropathy.
HostingRepository	PRIDE
AnnounceDate	2018-10-19
AnnouncementXML	Submission_2018-10-19_12:07:03.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Tamar Ziv
SpeciesList	scientific name: Danio rerio (Zebrafish) (Brachydanio rerio); NCBI TaxID: 7955;
ModificationList	S-carboxamidoethyl-L-cysteine; monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2018-02-20 01:50:00	ID requested
⏵ 1	2018-10-19 12:07:04	announced

Faria M, Ziv T, G, ó, mez-Canela C, Ben-Lulu S, Prats E, Novoa-Luna KA, Admon A, Pi, ñ, a B, Tauler R, G, ó, mez-Oliv, á, n LM, Rald, ú, a D, Acrylamide acute neurotoxicity in adult zebrafish. Sci Rep, 8(1):7918(2018) [pubmed]

curator keyword: Biomedical

submitter keyword: zebrafish, neurotoxicity, Shotgun proteomics

Demetrio Raldúa
contact affiliation	2IDAEA-CSIC, Jordi Girona 18, 08034, Barcelona, Spain
contact email	demetrio.raldua@idaea.csic.es
lab head
Tamar Ziv
contact affiliation	Technion
contact email	tamarz@tx.technion.ac.il
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2018/10/PXD008993

PRIDE project URI

• PRIDE
  1. PXD008993
     1. Label: PRIDE project
     2. Name: Acrylamide acute neurotoxicity in adult zebrafish