PXD008987

PXD008987 is an original dataset announced via ProteomeXchange.

Title	Chemoproteomic identification of molecular targets of antifungal prototypes, thiosemicarbazide and a camphene derivative of thiosemicarbazide, in Paracoccidioides brasiliensis
Description	Paracoccidioidomycosis (PCM) is a neglected human systemic disease caused by species of the genus Paracoccidioides. The disease attacks the host’s lungs and may disseminate to many other organs. Treatment involves amphotericin B, sulfadiazine, trimethoprim-sulfamethoxazole, itraconazole, ketoconazole, or fluconazole. The treatment duration is usually long, from 6 months to 2 years, and many adverse effects may occur in relation to the treatment; co-morbidities and poor treatment adherence have been noted. Therefore, the discovery of more effective and less toxic drugs is needed. Thiosemicarbazide (TSC) and a camphene derivative of thiosemicarbazide (TSC-C) were able to inhibit P. lutzii growth at a low dosage and were not toxic to fibroblast cells. In order to investigate the mode of action of those compounds, we used a chemoproteomic approach to determine which fungal proteins were bound to each of these compounds. The compounds were able to inhibit the activities of the enzymes formamidase and β-1,3-glucosidase and interfered in P. brasiliensis dimorphism. In comparison with the transcriptomic and proteomic data previously obtained by our group, we determined that TSC and TSC-C were multitarget compounds that exerted effects on the electron-transport chain and cell cycle regulation, increased ROS formation, inhibited proteasomes and peptidases, modulated glycolysis, lipid, protein and carbohydrate metabolisms, and caused suppressed the mycelium to yeast transition.
HostingRepository	PRIDE
AnnounceDate	2018-10-19
AnnouncementXML	Submission_2018-10-19_12:09:57.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Joyce Borba
SpeciesList	scientific name: Paracoccidioides brasiliensis (strain Pb18); NCBI TaxID: 502780;
ModificationList	No PTMs are included in the dataset
Instrument	ACQUITY UPLC

Revision	Datetime	Status	ChangeLog Entry
0	2018-02-20 01:29:46	ID requested
⏵ 1	2018-10-19 12:09:58	announced

Borba JVVB, Tauhata SBF, Oliveira CMA, Ferreira Marques M, Bail, ã, o AM, Soares CMA, Pereira M, Chemoproteomic identification of molecular targets of antifungal prototypes, thiosemicarbazide and a camphene derivative of thiosemicarbazide, in Paracoccidioides brasiliensis. PLoS One, 13(8):e0201948(2018) [pubmed]

curator keyword: Biomedical

submitter keyword: Paracoccidioidomycosis, chemoproteomic, thiosemicarbazide, camphene

Joyce Borba
contact affiliation	Universidade Federal de Goias
contact email	joycevillaverde@gmail.com
lab head
Joyce Borba
contact affiliation	Universidade Federal de Goiás
contact email	joycevillaverde@gmail.com
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD008987
     1. Label: PRIDE project
     2. Name: Chemoproteomic identification of molecular targets of antifungal prototypes, thiosemicarbazide and a camphene derivative of thiosemicarbazide, in Paracoccidioides brasiliensis