PXD008980

PXD008980 is an original dataset announced via ProteomeXchange.

Title	Time-resolved proteomics of Adenovirus infected cells
Description	To combat virus infections, which are major human killers, a deeper understanding of how viruses reprogram their hosts to create optimal production of progeny is needed. Most knowledge on the regulation of cellular gene expression during adenovirus infection is derived from studies of mRNA expression. Here, we investigated the changes in cellular protein expression during the early phase of adenovirus type 2 (Ad2) infection of primary human cells by stable isotope labeling in cell culture (SILAC) with subsequent liquid chromatography-high resolution tandem mass spectrometric (LC-MS/MS) analysis using a Q-Exactive Orbitrap instrument. Cells were in-depth evaluated 6 and 12 hours post infection (hpi) and two biological replicates were investigated using swapped labeling. In total, 2027 and 2150 proteins were quantified at 6 and 12 hpi, respectively. Among them, 431 and 544 were deregulated more than 1.5-fold at the two time points. For the deregulated proteins the change in protein expression was compared with that of late phase of infection (see PXD004095). Pathway analysis showed that De novo purine and pyrimidine biosynthesis, Glycolysis and Cytoskeletal regulation by Rho GTPase pathways are activated early during the infection, while the inactivation of the Integrin signalling pathway starts between 6 and 12 hpi. The transcription factor MYC was predicted to be activated with time, and the phosphopeptide analysis revealed the up-regulation of phosphosites related with glycolysis or cytoskeletal reorganization. These results complement the previous knowledge obtained from transcriptomic data, and show novel and specific aspects of how adenovirus influence host cell gene expression at the protein level.The results contribute to our understanding of host cell gene regulation during infection at a deeper level.
HostingRepository	PRIDE
AnnounceDate	2018-10-04
AnnouncementXML	Submission_2018-10-04_08:44:42.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Sara Lind
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606; scientific name: Human adenovirus C serotype 2 (HAdV-2) (Human adenovirus 2); NCBI TaxID: 10515;
ModificationList	phosphorylated residue; monohydroxylated residue; acetylated residue; iodoacetamide derivatized residue
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2018-02-19 05:34:25	ID requested
⏵ 1	2018-10-04 08:44:44	announced

Vald, é, s A, Zhao H, Pettersson U, Lind SB, Time-resolved proteomics of adenovirus infected cells. PLoS One, 13(9):e0204522(2018) [pubmed]

curator keyword: Biological, Biomedical

submitter keyword: Adenovirus type 2 infection, deregulation of host cell proteins, IMR-90, mass spectrometry, SILAC.

Sara Lind
contact affiliation	Uppsala University, Department of Chemistry-BMC, Analytical Chemistry
contact email	sara.lind@kemi.uu.se
lab head
Sara Lind
contact affiliation	Uppsala University
contact email	sara.lind@kemi.uu.se
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD008980
     1. Label: PRIDE project
     2. Name: Time-resolved proteomics of Adenovirus infected cells