PXD008979

PXD008979 is an original dataset announced via ProteomeXchange.

Title	CHEMICAL PROTEOMIC ANALYSIS OF ENDOCANNABINOID HYDROLASE ACTIVITY IN NIEMANN-PICK TYPE C MOUSE BRAIN
Description	The endocannabinoid system is considered to be an endogenous protective system in various neurodegenerative diseases. Niemann-Pick Type C is a neurodegenerative disease in which the role of the endocannabinoid system has not been studied yet. Here, we report the endocannabinoid hydrolase activity in brain proteomes of a Niemann-Pick type C mouse model as measured by activity-based protein profiling. Diacylglycerol lipase α, α/β-hydrolase domain-containing protein 4 (ABHD4), ABHD6, ABHD12, fatty acid amide hydrolase and monoacylglycerol lipase activities were quantified. Chemical proteomics showed no difference in endocannabinoid hydrolase activity in the brain of wildtype compared to Niemann-Pick C1 protein (NPC1) knockout mice. Three lysosomal serine hydrolases were identified with increased activity in NPC1 knockout mouse brain: retinoid-inducible serine carboxypeptidase, cathepsin A and palmitoyl-protein thioesterase 1, and we conclude that these might be interesting therapeutic targets for future validation studies.
HostingRepository	PRIDE
AnnounceDate	2018-04-19
AnnouncementXML	Submission_2018-11-21_10:07:16.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Bogdan Florea
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationList	monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Synapt MS; LTQ Orbitrap

Revision	Datetime	Status	ChangeLog Entry
0	2018-02-19 05:29:32	ID requested
1	2018-04-19 03:53:13	announced
⏵ 2	2018-11-21 10:07:17	announced	Updated publication reference for PubMed record(s): 30018533.

van Rooden EJ, van Esbroeck ACM, Baggelaar MP, Deng H, Florea BI, Marques ARA, Ottenhoff R, Boot RG, Overkleeft HS, Aerts JMFG, van der Stelt M, Chemical Proteomic Analysis of Serine Hydrolase Activity in Niemann-Pick Type C Mouse Brain. Front Neurosci, 12():440(2018) [pubmed]

curator keyword: Biological

submitter keyword: mouse, brain, niemann-pick type C, activity-based protein profiling, orbitrap, synapt

Mario van der Stelt
contact affiliation	Molecular Physiology, Leiden Institute for Chemistry, Leiden University, The Netherlands
contact email	m.van.der.stelt@chem.leidenuniv.nl
lab head
Bogdan Florea
contact affiliation	Leiden Institute for Chemistry
contact email	b.florea@chem.leidenuniv.nl
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD008979
     1. Label: PRIDE project
     2. Name: CHEMICAL PROTEOMIC ANALYSIS OF ENDOCANNABINOID HYDROLASE ACTIVITY IN NIEMANN-PICK TYPE C MOUSE BRAIN