PXD008977

PXD008977 is an original dataset announced via ProteomeXchange.

Title	Proteomes of leaf growing zones in rice genotypes with contrasting drought tolerance
Description	Plants require a distinctive cohort of enzymes to coordinate division and cell expansion. Proteomic analysis now enables interrogation of immature leaf bases where these processes occur. Hence we investigated proteins in tissues sampled from leaves of a drought-tolerant rice (IAC1131) to provide insights into the effect of soil drying on gene expression when compared with the drought-sensitive Nipponbare. Shoot growth zones were dissected to count dividing cells and extract protein for subsequent Tandem Mass Tags (TMT) quantitative proteomic analysis. Gene Ontology (GO) annotations of differentially expressed proteins provided insights into responses of Nipponbare and IAC1131 to drought. Soil drying did not affect the proportion of mitotic cells in IAC1131. More than 800 proteins across most functional categories were up-regulated in drought (and down-regulated on re-watering) in IAC1131, including those involved in organization of the meristem and subsequent cell formation. On the other hand, the proportion of dividing cells in Nipponbare was severely impaired during drought and fewer than 200 proteins responded in abundance when the growing zones underwent a drying cycle. However, those proteins involved in oxidation state and response to external stimuli were more likely to be upregulated by drought, even in Nipponbare.
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_04:43:15.834.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Yunqi Wu
SpeciesList	scientific name: Oryza sativa (Rice); NCBI TaxID: 4530;
ModificationList	monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2018-02-19 02:00:11	ID requested
1	2019-03-26 09:59:07	announced
⏵ 2	2024-10-22 04:43:24	announced	2024-10-22: Updated project metadata.

Wu Y, Mirzaei M, Pascovici D, Haynes PA, Atwell BJ, Proteomes of Leaf-Growing Zones in Rice Genotypes with Contrasting Drought Tolerance. Proteomics, 19(9):e1800310(2019) [pubmed]

10.1002/pmic.201800310;

curator keyword: Biological

submitter keyword: Rice, Growing zone, Plant proteomics,Abiotic stress, Drought stress

Paul A. Haynes
contact affiliation	Department of Molecular Sciences, Macquarie University, North Ryde, NSW, Australia
contact email	paul.haynes@mq.edu.au
lab head
Yunqi Wu
contact affiliation	Macquarie University
contact email	yunqi.wu@mq.edu.au
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2019/03/PXD008977

PRIDE project URI

• PRIDE
  1. PXD008977
     1. Label: PRIDE project
     2. Name: Proteomes of leaf growing zones in rice genotypes with contrasting drought tolerance