PXD008962

PXD008962 is an original dataset announced via ProteomeXchange.

Title	A regulatory cascade reprograms the ubiquitin ligase Ubr1 to enhance protein quality control during stress
Description	When faced with proteotoxic stress, cells mount adaptive responses to eliminate aberrant proteins. Adaptive responses enhance the cellular quality control machinery by increasing expression of protein folding and degradation factors, but it is unclear if and how this augmented machinery acquires new activities during stress. Here, we uncover a regulatory cascade in budding yeast that consists of the hydrophilin protein Roq1, the HtrA protease Ynm3 and the ubiquitin ligase Ubr1. Various stresses stimulate ROQ1 transcription. The Roq1 protein is cleaved by Ynm3. Cleaved Roq1 allosterically regulates Ubr1, transforming its substrate specificity. Altered substrate recognition by Ubr1 accelerates proteasomal degradation of misfolded and native proteins at the endoplasmic reticulum membrane and in the cytosol. We term this pathway stress-induced homeostatically regulated protein degradation (SHRED) and propose that it promotes physiological adaptation by reprogramming a key component of the cellular quality control machinery.
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_04:42:37.998.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Daniel Itzhak
SpeciesList	scientific name: Saccharomyces cerevisiae (Baker's yeast); NCBI TaxID: 4932;
ModificationList	monohydroxylated residue; acetylated residue; iodoacetamide derivatized residue
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2018-02-15 02:28:45	ID requested
1	2018-06-06 06:50:20	announced
2	2019-02-15 09:05:35	announced	Updated publication reference for PubMed record(s): 29861160.
⏵ 3	2024-10-22 04:42:38	announced	2024-10-22: Updated project metadata.

10.1016/j.molcel.2018.04.027;

Szoradi T, Schaeff K, Garcia-Rivera EM, Itzhak DN, Schmidt RM, Bircham PW, Leiss K, Diaz-Miyar J, Chen VK, Muzzey D, Borner GHH, Schuck S, SHRED Is a Regulatory Cascade that Reprograms Ubr1 Substrate Specificity for Enhanced Protein Quality Control during Stress. Mol Cell, 70(6):1025-1037.e5(2018) [pubmed]

curator keyword: Biological

submitter keyword: LC-MSMS,Yeast, Qexactive HF, Orbitrap

Georg Borner
contact affiliation	Max Planck Institute of Biochemistry
contact email	borner@biochem.mpg.de
lab head
Daniel Itzhak
contact affiliation	Max Planck Institute of Biochemistry
contact email	itzhak@biochem.mpg.de
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD008962
     1. Label: PRIDE project
     2. Name: A regulatory cascade reprograms the ubiquitin ligase Ubr1 to enhance protein quality control during stress