PXD008954

PXD008954 is an original dataset announced via ProteomeXchange.

Title	Comparative proteomics of three species of ammonia-oxidizing bacteria under ammonia starved and ammonia replete conditions
Description	In this experiment, we used advanced proteomics techniques to discern differences in energy allocation between three strains of ammonia oxidizing bacteria: Nitrosomonas europaea, Nitrosomonas ureae, and Nitrosospira multiformis, during ammonia starved and ammonia replete conditions. Replicate cultures in late log phase from the three strains were starved of ammonia for 24 hours and compared to replicate control cultures grown for the same period. All three species were grown with three biological replicates for each condition and species with the exception of two replicates from the N. ureae starved cultures due to sample processing loss. This study has, to our knowledge, produced the first complete proteomes of Nitrosospira multiformis and Nitrosomonas ureae.
HostingRepository	PRIDE
AnnounceDate	2023-11-14
AnnouncementXML	Submission_2023-11-14_08:49:26.278.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Jackie Zorz
SpeciesList	scientific name: Nitrosospira multiformis ATCC 25196; NCBI TaxID: 323848; scientific name: Nitrosomonas ureae; NCBI TaxID: 44577; scientific name: Nitrosomonas europaea ATCC 19718; NCBI TaxID: 228410;
ModificationList	monohydroxylated residue; acetylated residue; iodoacetamide derivatized residue
Instrument	Q Exactive Plus

Revision	Datetime	Status	ChangeLog Entry
0	2018-02-14 01:39:11	ID requested
1	2018-05-16 00:48:35	announced
2	2018-05-28 00:44:50	announced	Updated publication reference for DOI(s): 10.3389/fmicb.2018.00938.
3	2018-06-07 00:19:25	announced	Updated publication reference for DOI(s): 10.3389/fmicb.2018.00938.
⏵ 4	2023-11-14 08:49:28	announced	2023-11-14: Updated project metadata.

Zorz JK, Kozlowski JA, Stein LY, Strous M, Kleiner M, Comparative Proteomics of Three Species of Ammonia-Oxidizing Bacteria. Front Microbiol, 9():938(2018) [pubmed]

curator keyword: Biological

submitter keyword: Nitrosomonas,1D-LC, comparative proteomics, proteomes, Nitrosospira, ammonia oxidizing bacteria, nitrogen cycle, nitrification, ammonia

Manuel Kleiner
contact affiliation	Department of Plant and Microbial Biology, North Carolina State University, Raleigh, North Carolina, USA
contact email	mkleine@ncsu.edu
lab head
Jackie Zorz
contact affiliation	University of Calgary
contact email	jacqueline.zorz@ucalgary.ca
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2018/05/PXD008954

PRIDE project URI

• PRIDE
  1. PXD008954
     1. Label: PRIDE project
     2. Name: Comparative proteomics of three species of ammonia-oxidizing bacteria under ammonia starved and ammonia replete conditions