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DataSet Summary

  • HostingRepository: PRIDE
  • AnnounceDate: 2018-05-16
  • AnnouncementXML: Submission_2018-06-07_00:19:24.xml
  • DigitalObjectIdentifier:
  • ReviewLevel: Peer-reviewed dataset
  • DatasetOrigin: Original data
  • RepositorySupport: Unsupported dataset by repository
  • PrimarySubmitter: Jackie Zorz
  • Title: Comparative proteomics of three species of ammonia-oxidizing bacteria under ammonia starved and ammonia replete conditions
  • Description: In this experiment, we used advanced proteomics techniques to discern differences in energy allocation between three strains of ammonia oxidizing bacteria: Nitrosomonas europaea, Nitrosomonas ureae, and Nitrosospira multiformis, during ammonia starved and ammonia replete conditions. Replicate cultures in late log phase from the three strains were starved of ammonia for 24 hours and compared to replicate control cultures grown for the same period. All three species were grown with three biological replicates for each condition and species with the exception of two replicates from the N. ureae starved cultures due to sample processing loss. This study has, to our knowledge, produced the first complete proteomes of Nitrosospira multiformis and Nitrosomonas ureae.
  • SpeciesList: scientific name: Nitrosospira multiformis ATCC 25196; NCBI TaxID: 323848; scientific name: Nitrosomonas ureae; NCBI TaxID: 44577; scientific name: Nitrosomonas europaea ATCC 19718; NCBI TaxID: 228410;
  • ModificationList: monohydroxylated residue; acetylated residue; iodoacetamide derivatized residue
  • Instrument: Q Exactive Plus

Dataset History

VersionDatetimeStatusChangeLog Entry
02018-02-14 01:39:11ID requested
12018-05-16 00:48:35announced
22018-05-28 00:44:50announcedUpdated publication reference for DOI(s): 10.3389/fmicb.2018.00938.
32018-06-07 00:19:25announcedUpdated publication reference for DOI(s): 10.3389/fmicb.2018.00938.

Publication List

  1. Zorz JK, Kozlowski JA, Stein LY, Strous M, Kleiner M, Comparative Proteomics of Three Species of Ammonia-Oxidizing Bacteria. Front Microbiol, 9():938(2018) [pubmed]
  2. 10.3389/fmicb.2018.00938;

Keyword List

  1. curator keyword: Biological
  2. submitter keyword: 1D-LC, proteomes, comparative proteomics, ammonia oxidizing bacteria, Nitrosomonas, Nitrosospira, nitrogen cycle, ammonia, nitrification

Contact List

    Manuel Kleiner
    • contact affiliation: Department of Plant and Microbial Biology, North Carolina State University, Raleigh, North Carolina, USA
    • contact email: mkleine@ncsu.edu
    • lab head:
    Jackie Zorz
    • contact affiliation: University of Calgary
    • contact email: jacqueline.zorz@ucalgary.ca
    • dataset submitter:

Full Dataset Link List

  1. Dataset FTP location
  2. PRIDE project URI
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