PXD008950

PXD008950 is an original dataset announced via ProteomeXchange.

Title	Lipid droplet proteomics of THP1 macrophages infected with Mycobacterium tuberculosis
Description	Growing evidence suggests the importance of lipid metabolism in pathogenesis of tuberculosis. Neutral lipids form the majority of lipids in caseous granulomas characteristic of tuberculosis. Macrophage lipid droplets form the store house of these lipids, yet infection induced changes in the proteome of these dynamic organelles remains elusive. Here we employed quantitative proteomics to identify alterations induced upon infection with live Mycobacterium tuberculosis in comparison with heat killed bacilli and uninfected macrophages. Association of specific proteins coupled with lysosomal function was found to be increased upon infection with live M. tuberculosis. Biochemical and microscopy based evidence validated the enrichment of the small GTPase Arl8b, the guanine nucleotide effector LAMTOR1, and the scavenger receptor SCARB2 on lipid droplets during live Mtb infection. We validated the localization of Arl8b on lipid droplets using super-resolution microscopy. Increased abundance of these lysosomal proteins on lipid droplets upon infection with live Mtb suggests active manipulation of the host lipid droplets by the pathogen. Furthermore, depletion of lipid droplets upon stable knockdown of diacylglycerol O-acyltransferase led to reduction in lysosomal acidification of infected cells, suggesting an active role of lipid droplets in lysosomal function during infection.
HostingRepository	PRIDE
AnnounceDate	2019-11-14
AnnouncementXML	Submission_2019-11-14_00:48:35.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Sheetal Gandotra
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	TMT6plex-126 reporter+balance reagent acylated residue; monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Orbitrap Fusion

Revision	Datetime	Status	ChangeLog Entry
0	2018-02-14 01:28:57	ID requested
⏵ 1	2019-11-14 00:55:13	announced

Menon D, Singh K, Pinto SM, Nandy A, Jaisinghani N, Kutum R, Dash D, Prasad TSK, Gandotra S, Quantitative Lipid Droplet Proteomics Reveals Mycobacterium tuberculosis Induced Alterations in Macrophage Response to Infection. ACS Infect Dis, 5(4):559-569(2019) [pubmed]

curator keyword: Biomedical

submitter keyword: Tuberculosis, lipid droplets, subcellular proteomics, Orbitrap Fusion, lysosomes, macrophage

Sheetal Gandotra
contact affiliation	CSIR-Institute of Genomics and Integrative Biology
contact email	sheetal.gandotra@igib.res.in
lab head
Sheetal Gandotra
contact affiliation	CSIR-IGIB
contact email	sheetal.gandotra@igib.res.in
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2019/11/PXD008950

PRIDE project URI

• PRIDE
  1. PXD008950
     1. Label: PRIDE project
     2. Name: Lipid droplet proteomics of THP1 macrophages infected with Mycobacterium tuberculosis