PXD008925

PXD008925 is an original dataset announced via ProteomeXchange.

Title	MOLT-4 radiosensitization by VE-821
Description	DNA damage induction by either radio- or chemo-therapy has been the most widely used approach in cancer therapy, exploiting the genomic instability of cancer cells. A promising strategy takes advantage of tumour specific abnormalities in DNA damage response. Inhibition of the ATR/Chk1 pathway has been shown to be synthetically lethal in cells with high levels of oncogene-induced replication stress and in p53- or ATM- deficient cells. In the presented study, we aimed to elucidate molecular mechanisms underlying radiosensitization of MOLT-4 cells by VE-821, a higly potent and specific inhibitor of ATR. We combined multiple approaches: cell biology techniques to reveal the inhibitor-induced phenotypes, and quantitative proteomics, phosphoproteomics, and metabolomics to comprehensively describe drug-induced changes in irradiated cells.
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_04:43:04.171.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Barbora Salovska
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	phosphorylated residue
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2018-02-12 01:33:22	ID requested
1	2018-07-16 04:00:54	announced
⏵ 2	2024-10-22 04:43:12	announced	2024-10-22: Updated project metadata.

10.1371/journal.pone.0199349;

Š, alovsk, á B, Jane, č, kov, á H, Fabrik I, Karl, í, kov, á R, Č, ech, á, kov, á L, Ondrej M, Link M, Friedeck, ý D, Tich, ý A, Radio-sensitizing effects of VE-821 and beyond: Distinct phosphoproteomic and metabolomic changes after ATR inhibition in irradiated MOLT-4 cells. PLoS One, 13(7):e0199349(2018) [pubmed]

curator keyword: Biomedical

submitter keyword: ionizing radiation

small-molecule kinase inhibitors

VE-821

ATR kinase

DNA damage response

radio-sensitization

quantitative shotgun phosphoproteomics

titanium dioxide chromatography

SILAC

leukemia

MOLT-4 cells

Aleš Tichý
contact affiliation	Department of Radiobiology, Faculty of Military Health Sciences in Hradec Králové, University of Defence in Brno, Hradec Králové, Czech Republic
contact email	ales.tichy@unob.cz
lab head
Barbora Salovska
contact affiliation	Institute of Molecular Genetics of the Czech Academy of Sciences
contact email	salovska@img.cas.cz
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2018/07/PXD008925

PRIDE project URI

• PRIDE
  1. PXD008925
     1. Label: PRIDE project
     2. Name: MOLT-4 radiosensitization by VE-821