PXD008924

PXD008924 is an original dataset announced via ProteomeXchange.

Title	Time series and steady state proteomics of Pseudomonas aeruginosa phenazine mutants
Description	Microbes in biofilms face the challenge of substrate limitation. In particular, cells in Pseudomonas aeruginosa biofilms growing in the laboratory or during host colonization often become limited for oxygen. Previously we found that phenazines, antibiotics produced by P. aeruginosa, balance the intracellular redox state for cells in biofilms. Here, we show that genes involved in denitrification are induced in phenazine-null (Δphz) mutant biofilms grown under an aerobic atmosphere, even in the absence of nitrate. This finding suggests that resident cells employ a bet-hedging strategy to anticipate the potential availability of nitrate and counterbalance their highly reduced redox state. Consistent with our previous characterization of aerobically-grown colonies supplemented with nitrate, we find that the pathway that is induced in Δphz colonies combines the nitrate reductase activity of the periplasmic enzyme Nap with downstream reduction of nitrite to nitrogen gas catalyzed by the enzymes Nir, Nor, and Nos. This regulatory relationship differs from the denitrification pathway that functions under anaerobic growth with nitrate as the terminal electron acceptor, which depends on the membrane-associated nitrate reductase Nar. We identify sequences in the promoter regions of the nap and nir operons that are required for the effects of phenazines on expression. We also show that specific phenazines have differential effects on nap gene expression. Finally, we provide evidence that individual steps of the denitrification pathway are catalyzed at different depths within aerobically grown biofilms, suggesting metabolic cross-feeding between community subpopulations. (corresponding publication)
HostingRepository	PRIDE
AnnounceDate	2018-10-19
AnnouncementXML	Submission_2018-10-19_12:58:54.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Christine Vogel
SpeciesList	scientific name: Pseudomonas aeruginosa PA14-RN6; NCBI TaxID: 910263;
ModificationList	iodoacetamide derivatized residue
Instrument	LTQ Orbitrap Elite

Revision	Datetime	Status	ChangeLog Entry
0	2018-02-12 01:21:22	ID requested
⏵ 1	2018-10-19 12:58:55	announced

Lin YC, Sekedat MD, Cornell WC, Silva GM, Okegbe C, Price-Whelan A, Vogel C, Dietrich LEP, Phenazines Regulate Nap-Dependent Denitrification in Pseudomonas aeruginosa Biofilms. J Bacteriol, 200(9):(2018) [pubmed]

curator keyword: Biological

submitter keyword: biofilm

phenazines

denitrification

Pseudomonas aeruginosa

Christine Vogel
contact affiliation	New York University
contact email	cvogel@nyu.edu
lab head
Christine Vogel
contact affiliation	New York University
contact email	cvogel@nyu.edu
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD008924
     1. Label: PRIDE project
     2. Name: Time series and steady state proteomics of Pseudomonas aeruginosa phenazine mutants