PXD008901

PXD008901 is an original dataset announced via ProteomeXchange.

Title	Change of Ranibizumab-induced Human Vitreous Protein Profile in Patients with Proliferative Diabetic Retinopathy Based on Proteomics Analysis
Description	Background: Preoperative treatment of anti-vascular endothelial growth factor (VEGF) agents is extensively used in proliferative diabetic retinopathy (PDR), but the molecular mechanism is not fully understood. The objective of this research is to observe change of protein profile induced by Ranibizumab (an anti-VEGF agent) in vitreous humor from PDR patients and reveal the effects of anti-VEGF treatment on PDR. Methods: A proteomic method was used to identify differentially expressed proteins in vitreous humor. Untreated PDR patients were defined as PDR group, while those who treated with intravitreal injection of ranibizumab (IVR) were defined as IVR. Gene Ontology (GO) annotation and REACTOME pathways were obtained from DAVID Bioinformatics Resources. Intravitreal level of apolipoprotein C-I (APOC1), serpin peptidase inhibitor clade A member 5 (SERPINA5), tissue inhibitor of metalloproteinases (TIMP2), and keratin 1 (KRT1) were determined by enzyme-linked immuno sorbent assay (ELISA). Results: 339 differentially expressed proteins were identified in response to IVR. The most notable GO annotation describes the altered proteins was “innate immune response”. The most notable REACTOME pathway was “platelet degranulation”. ELISA result showed increased level of APOC1, SERPINA5, KRT1 and a decreased level of TIMP2 in PDR group compared with IVR. Conclusions: In addition to decreasing VEGF level, Ranibizumab is associated with change of human vitreous protein profile in patients with PDR, in which the differential proteins are involved in immune response, platelet degranulation, complement activation etc., suggesting that the effects of VEGF are involved in these signaling pathways.
HostingRepository	PRIDE
AnnounceDate	2024-02-12
AnnouncementXML	Submission_2024-02-12_09:03:44.638.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Chen Zou
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	No PTMs are included in the dataset
Instrument	Thermo Fisher Scientific instrument model

Revision	Datetime	Status	ChangeLog Entry
0	2018-02-08 03:43:22	ID requested
1	2018-10-19 13:01:54	announced
⏵ 2	2024-02-12 09:03:46	announced	2024-02-12: Updated project metadata.

10.1186/s12014-018-9187-z;

Zou C, Han C, Zhao M, Yu J, Bai L, Yao Y, Gao S, Cao H, Zheng Z, Change of ranibizumab-induced human vitreous protein profile in patients with proliferative diabetic retinopathy based on proteomics analysis. Clin Proteomics, 15():12(2018) [pubmed]

curator keyword: Biomedical

submitter keyword: proteomics

Proliferative Diabetic Retinopathy

Ranibizumab

Zhi Zheng
contact affiliation	Department of Ophthalmology, Shanghai General Hospital; Shanghai Key Laboratory of Ocular Fundus Disease; Shanghai Engineering Center for Visual Science and Photomedicine, No. 100 Haining Road, Shanghai 200080, China.
contact email	zzheng88@sjtu.edu.cn
lab head
Chen Zou
contact affiliation	Department of Ophthalmology, Shanghai General Hospital; Shanghai Key Laboratory of Ocular Fundus Disease; Shanghai Engineering Center for Visual Science and Photomedicine, No. 100 Haining Road, Shanghai 200080, China.
contact email	chenzou0814@foxmail.com
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2018/10/PXD008901

PRIDE project URI

• PRIDE
  1. PXD008901
     1. Label: PRIDE project
     2. Name: Change of Ranibizumab-induced Human Vitreous Protein Profile in Patients with Proliferative Diabetic Retinopathy Based on Proteomics Analysis