PXD008900

PXD008900 is an original dataset announced via ProteomeXchange.

Title	Early mannitol-triggered changes in the Arabidopsis leaf (phospho)proteome
Description	Drought is one of the most detrimental environmental stresses to which plants are exposed. Especially mild drought is relevant to agriculture and significantly affects plant growth and development. In plant research, mannitol is often used to mimic drought stress and study the resulting responses. In growing leaf tissue of plants exposed to mannitol-induced stress, a highly-interconnected gene regulatory network is induced. However, early signaling and associated protein phosphorylation events that likely precede part of these transcriptional changes are largely unknown. Here, we performed a full proteome and phosphoproteome analysis on growing leaf tissue of Arabidopsis plants exposed to mild mannitol-induced stress and captured the fast (within the first half hour) events associated with this stress. Based on this in-depth data analysis, 167 and 172 differentially abundant and unique proteins and phosphorylated sites were found back, respectively. Finally, we identified H(+)-ATPASE 2 (AHA2) and CYSTEINE-RICH REPEAT SECRETORY PROTEIN 38 (CRRSP38) as novel regulators of shoot growth under osmotic stress.
HostingRepository	PRIDE
AnnounceDate	2018-10-19
AnnouncementXML	Submission_2018-10-19_13:04:53.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Natalia Nikonorova
SpeciesList	scientific name: Arabidopsis thaliana (Mouse-ear cress); NCBI TaxID: 3702;
ModificationList	phosphorylated residue; monohydroxylated residue; acetylated residue; iodoacetamide derivatized residue
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2018-02-08 03:39:46	ID requested
⏵ 1	2018-10-19 13:04:56	announced

Nikonorova N, Van den Broeck L, Zhu S, van de Cotte B, Dubois M, Gevaert K, Inz, é D, De Smet I, Early mannitol-triggered changes in the Arabidopsis leaf (phospho)proteome reveal growth regulators. J Exp Bot, 69(19):4591-4607(2018) [pubmed]

curator keyword: Biological

submitter keyword: Signalling, Phosphoproteome, Mild osmotic stress, Arabidopsis thaliana

Ive De Smet
contact affiliation	Ghent University, Department of Plant Biotechnology and Bioinformatics, 9052 Ghent, Belgium VIB Center for Plant Systems Biology, 9052 Ghent, Belgium
contact email	ivsme@psb.vib-ugent.be
lab head
Natalia Nikonorova
contact affiliation	Ghent University
contact email	nikonorova.nat@gmail.com
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2018/10/PXD008900

PRIDE project URI

• PRIDE
  1. PXD008900
     1. Label: PRIDE project
     2. Name: Early mannitol-triggered changes in the Arabidopsis leaf (phospho)proteome