PXD008899 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Re-analysis of Hela phosphoproteome (PXD000612) with TagGraph |
Description | Thousands of protein post-translational modifications (PTMs) dynamically impact nearly all cellular functions. Although mass spectrometry is suited to PTM identification, it has historically been biased towards a few with established enrichment procedures. To measure all possible PTMs across diverse proteomes, software must overcome two fundamental challenges: intractably large search spaces and difficulty distinguishing correct from incorrect identifications. Here, we describe TagGraph, software that overcomes both challenges with a string-based search method that is orders of magnitude faster than current approaches, and a probabilistic validation model optimized for PTM assignments. When applied to a human proteome map, TagGraph triples confident identifications while revealing thousands of modification types spanning nearly one million sites across the proteome. We show new contexts for highly abundant yet understudied PTMs such as proline hydroxylation. TagGraph expands our ability to survey the full proteomic landscape of PTMs, shedding new light on their tissue-specific functions. |
HostingRepository | PRIDE |
AnnounceDate | 2019-03-12 |
AnnouncementXML | Submission_2019-04-04_00:02:33.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Joshua Elias |
SpeciesList | scientific name: Homo sapiens (Human); NCBI TaxID: 9606; |
ModificationList | phosphorylated residue |
Instrument | Q Exactive |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2018-02-08 03:19:48 | ID requested | |
1 | 2019-03-11 20:53:45 | announced | |
⏵ 2 | 2019-04-04 00:02:34 | announced | Updated publication reference for PubMed record(s): 30936560. |
Publication List
Devabhaktuni A, Lin S, Zhang L, Swaminathan K, Gonzalez CG, Olsson N, Pearlman SM, Rawson K, Elias JE, TagGraph reveals vast protein modification landscapes from large tandem mass spectrometry datasets. Nat Biotechnol, 37(4):469-479(2019) [pubmed] |
Keyword List
curator keyword: Biological |
submitter keyword: TagGraph, Hela, PTM, Posphorylation |
Contact List
Joshua E Elias |
contact affiliation | Dept of Chemical & Systems Biology, Stanford University, Stanford CA 94305 |
contact email | josh.elias@stanford.edu |
lab head | |
Joshua Elias |
contact affiliation | Chemical & Systems Biology |
contact email | josh.elias@stanford.edu |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2019/03/PXD008899 |
PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD008899
- Label: PRIDE project
- Name: Re-analysis of Hela phosphoproteome (PXD000612) with TagGraph