PXD008897

PXD008897 is an original dataset announced via ProteomeXchange.

Title	GFI1 facilitates efficient DNA repair by regulating PRMT1-dependent methylation of MRE11 and 53BP1
Description	GFI1 is a transcriptional regulator expressed in lymphoid cells, and an "oncorequisite" factor required for development and maintenance of T-lymphoid leukemia. GFI1 deletion causes hypersensitivity to ionizing radiation, for which the molecular mechanism remains unknown. Here, we demonstrate that GFI1 is required in T cells for the regulation of key DNA damage signalling and repair proteins. Specifically, GFI1 interacts with the arginine methyltransferase PRMT1 and its substrates MRE11 and 53BP1. We demonstrate that GFI1 enables PRMT1 to bind and methylate MRE11 and 53BP1, which is necessary for their function in the DNA damage response. Thus, our results provide evidence that GFI1 can adopt non-transcriptional roles, mediating the post-translational modification of proteins involved in DNA repair. These findings have direct implications for treatment responses in tumours overexpressing GFI1 and suggest that GFI1's activity may be a therapeutic target in these malignancies.
HostingRepository	PRIDE
AnnounceDate	2018-10-19
AnnouncementXML	Submission_2018-10-19_13:05:37.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD008897
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Charles Vadnais
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	Oxidation; Carbamidomethyl
Instrument	LTQ Orbitrap Velos

Revision	Datetime	Status	ChangeLog Entry
0	2018-02-08 01:49:44	ID requested
⏵ 1	2018-10-19 13:05:39	announced

Vadnais C, Chen R, Fraszczak J, Yu Z, Boulais J, Pinder J, Frank D, Khandanpour C, H, é, bert J, Dellaire G, C, ô, t, é JF, Richard S, Orthwein A, Drobetsky E, M, ö, r, ö, y T, GFI1 facilitates efficient DNA repair by regulating PRMT1 dependent methylation of MRE11 and 53BP1. Nat Commun, 9(1):1418(2018) [pubmed]

curator keyword: Biological

submitter keyword: DNA repair GFI1 PRMT1 methylation MRE11 53bp1

Tarik Moroy
contact affiliation	Institut de Recherches Cliniques de Montréal, IRCM, Montréal, QC, H2W 1R7, Canada
contact email	Tarik.Moroy@ircm.qc.ca
lab head
Charles Vadnais
contact affiliation	Institut de Recherches Cliniques de Montréal
contact email	charles.vadnais@ircm.qc.ca
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2018/10/PXD008897

PRIDE project URI

• PRIDE
  1. PXD008897
     1. Label: PRIDE project
     2. Name: GFI1 facilitates efficient DNA repair by regulating PRMT1-dependent methylation of MRE11 and 53BP1