PXD008891

PXD008891 is an original dataset announced via ProteomeXchange.

Title	Multiplex Biomarker Screening Assay for Urinary Extracellular Vesicles Study: A Targeted Label-Free Proteomic Approach
Description	The recent advance in targeted label-free proteomics, SWATH-MS, can provide consistent protein detection and reproducible protein quantitation, which is a considerable advantage for biomarker study of urinary exosome-enriched extracellular vesicles (EVs). We developed a SWATH-MS workflow with a curated spectral library of 1,073 targets. Application of the workflow across nine replicates of three sample types (EVs, microvesicles (MVs) and urine proteins (UP)) resulting in the quantitation of 842 proteins. The median-coefficient of variation of the 842 proteins in the EV sample was 7.6%, indicating excellent reproducibility. Data analysis showed common EV markers, (i.e. CD9, CD63, ALIX, TSG101 and HSP70) were enriched in urinary EVs as compared to MV and UP samples. Further development and applicationof this SWATH-MS workflow to a variety of kidney diseases may allow for new and robust avenues for biomarker identification and validation for clinical use.
HostingRepository	PRIDE
AnnounceDate	2018-10-11
AnnouncementXML	Submission_2018-10-17_03:22:20.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Somchai Chutipongtanate
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	iodoacetamide derivatized residue
Instrument	TripleTOF 5600

Revision	Datetime	Status	ChangeLog Entry
0	2018-02-08 01:10:13	ID requested
1	2018-10-11 09:42:45	announced
⏵ 2	2018-10-17 03:22:22	announced	Updated publication reference for PubMed record(s): 30301925.

Chutipongtanate S, Greis KD, Multiplex Biomarker Screening Assay for Urinary Extracellular Vesicles Study: A Targeted Label-Free Proteomic Approach. Sci Rep, 8(1):15039(2018) [pubmed]

curator keyword: Biomedical

submitter keyword: Biomarker

Exosomes

Human urine

Microvesicles

Post-acquisition data extraction

proteome

Kenneth D. Greis
contact affiliation	UC Proteomics Laboratory, Department of Cancer Biology, University of Cincinnati College of Medicine, Cincinnati, Ohio, United States.
contact email	ken.greis@uc.edu
lab head
Somchai Chutipongtanate
contact affiliation	University of Cincinnati, Cincinnati, Ohio
contact email	schuti.rama@gmail.com
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2018/10/PXD008891

PRIDE project URI

• PRIDE
  1. PXD008891
     1. Label: PRIDE project
     2. Name: Multiplex Biomarker Screening Assay for Urinary Extracellular Vesicles Study: A Targeted Label-Free Proteomic Approach