PXD008890

PXD008890 is an original dataset announced via ProteomeXchange.

Dataset Summary

Title	Retinal-cysteine adduct in Opn5L1
Description	Targeted analysis of retinal-cysteine adduct in Opsin 5 like-1 (Opn5L1) protein Chicken Opn5L1 E177K/Q192K mutant was expressed in HEK293T, regenerated by all-trans-retinal, solubilized by dodecyl maltoside, and purified using Rho1D4 antibody-conjugated Sephalose. Semi-quantitative targeted analyses of trypsinized Opn5L1 with or without light irradiation were carried out using reverse phase LC-ESI-MSn to detect light-dependent formation of retinal-cysteine adduct. The results were analyzed by Mass++, mMass and Igor Pro softwares.
HostingRepository	jPOST
AnnounceDate	2018-02-15
AnnouncementXML	Submission_2022-09-18_02:20:16.289.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Keita Sato
SpeciesList	scientific name: Gallus gallus; NCBI TaxID: 9031;
ModificationList	S-carboxamidomethyl-L-cysteine; unknown modification
Instrument	LTQ Orbitrap

Dataset History

Revision	Datetime	Status	ChangeLog Entry
0	2018-02-07 23:32:23	ID requested
1	2018-02-14 07:00:10	announced
2	2018-05-19 23:40:13	announced	Updated PubMed.
3	2018-09-19 00:48:23	announced	Updated CV, FTP location and Modification list.
4	2021-02-04 16:57:50	announced	2021-02-04: Updated FTP location.
⏵ 5	2022-09-18 02:20:17	announced	2022-09-18: Updated FTP location.

Publication List

Sato K, Yamashita T, Ohuchi H, Takeuchi A, Gotoh H, Ono K, Mizuno M, Mizutani Y, Tomonari S, Sakai K, Imamoto Y, Wada A, Shichida Y, Opn5L1 is a retinal receptor that behaves as a reverse and self-regenerating photoreceptor. Nat Commun, 9(1):1255(2018) [pubmed]

Sato K, Yamashita T, Ohuchi H, Takeuchi A, Gotoh H, Ono K, Mizuno M, Mizutani Y, Tomonari S, Sakai K, Imamoto Y, Wada A, Shichida Y, Opn5L1 is a retinal receptor that behaves as a reverse and self-regenerating photoreceptor. Nat Commun, 9(1):1255(2018) [pubmed]

Keyword List

submitter keyword: label-free, targeted analysis

submitter keyword: label-free, targeted analysis

Contact List

Yoshinori Shichida
lab head
Keita Sato
contact affiliation	Okayama University
dataset submitter

Full Dataset Link List

jPOST dataset URI
Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.jpostdb.org/JPST000386/

jPOST dataset URI

Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.jpostdb.org/JPST000386/