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PXD008881

PXD008881 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleBottom-up and top-down LC-MS/MS of ribosomal purifications
DescriptionRibosomal purifications of spinach 70S ribosome and human 40S and 60S ribosomal subunits were analyzed with bottom-up LC-MS/MS to identify proteins comprising purified complexes as well as co-purified proteins. In order to assess and quantify proteoforms of ribosomal proteins in ribosomal purifications we employed top-down LC-MS/MS techniques with optimized methods, wherein apart from standard parameters (e.g. collision voltage and dynamic exclusion time) high-resolution and low-resolution were alternately employed in full MS mode.
HostingRepositoryPRIDE
AnnounceDate2018-06-26
AnnouncementXMLSubmission_2018-07-02_07:18:38.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterSem Tamara
SpeciesList scientific name: Escherichia coli; NCBI TaxID: 562; scientific name: Spinacia oleracea; NCBI TaxID: 3562; scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationListmonomethylated residue; phosphorylated residue; monohydroxylated residue; acetylated residue; dimethylated residue; iodoacetamide derivatized residue
InstrumentQ Exactive HF
Dataset History
RevisionDatetimeStatusChangeLog Entry
02018-02-07 01:41:20ID requested
12018-06-26 06:37:05announced
22018-07-02 07:18:40announcedUpdated publication reference for PubMed record(s): 29950687.
Publication List
van de Waterbeemd M, Tamara S, Fort KL, Damoc E, Franc V, Bieri P, Itten M, Makarov A, Ban N, Heck AJR, Dissecting ribosomal particles throughout the kingdoms of life using advanced hybrid mass spectrometry methods. Nat Commun, 9(1):2493(2018) [pubmed]
Keyword List
curator keyword: Biological
submitter keyword: ribosome, top-down, bottom-up
Contact List
Albert J. R. Heck
contact affiliationBiomolecular Mass Spectrometry and Proteomics, Bijvoet Center for Biomolecular Research and Utrecht Institute for Pharmaceutical Sciences, Utrecht University, Utrecht, the Netherlands
contact emaila.j.r.heck@uu.nl
lab head
Sem Tamara
contact affiliationUtrecht University
contact emails.tamara@uu.nl
dataset submitter
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Dataset FTP location
PRIDE project URI
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