PXD008874

PXD008874 is an original dataset announced via ProteomeXchange.

Title	Eukaryotic translation initiation factor 5A regulates autophagy via translation of ATG3
Description	Autophagy is an essential catabolic process responsible for recycling of intracellular material and preserving cellular fidelity. Key to the autophagy pathway is the ubiquitin-like conjugation system mediating lipidation of Atg8 proteins and their anchoring to autophagosomal membranes. While regulation of autophagy has been characterized at the level of transcription, protein interactions and post-translational modifications, its translational regulation remains elusive. Here we describe a novel regulatory axis of autophagy at the translational level, guided by the conserved eukaryotic translation factor eIF5A. Identified from a high-throughput screen, we find that eIF5A is required for lipidation of LC3B and its paralogs and promotes autophagosome formation. This feature is evolutionarily conserved and results from the translation of the E2-like ATG3 protein. Mechanistically, we identify an amino acid motif in ATG3 causing eIF5A-dependency for its efficient translation. Our study identifies a key regulatory mechanism of autophagosome formation and demonstrates the impact of translation in the regulation autophagy.
HostingRepository	PRIDE
AnnounceDate	2018-04-27
AnnouncementXML	Submission_2018-04-27_04:08:25.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Michal Lubas
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	No PTMs are included in the dataset
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2018-02-06 08:19:45	ID requested
⏵ 1	2018-04-27 04:08:26	announced

Dataset with its publication pending

curator keyword: Biological

submitter keyword: eIF5A, LC-MS, newly synthesized proteins

Anders Lund
contact affiliation	Biotech Research and Innovation Centre, University of Copenhagen, Denmark
contact email	anders.lund@bric.ku.dk
lab head
Michal Lubas
contact affiliation	University of Copenhagen
contact email	michal.lubas@bric.ku.dk
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2018/04/PXD008874

PRIDE project URI

• PRIDE
  1. PXD008874
     1. Label: PRIDE project
     2. Name: Eukaryotic translation initiation factor 5A regulates autophagy via translation of ATG3