PXD008871

PXD008871 is an original dataset announced via ProteomeXchange.

Title	Comparative proteomic analysis provides novel insights into the regulation mechanism underlying the fruit ripening process in papaya (Carica papaya L.)
Description	Papaya (Carica papaya L.) is a typical climacteric fruit, undergoing massive physico-chemical changes during ripening. Although papaya is widely cultivated and consumed, few studies have characterized the variations in metabolism during its ripening process at the proteasome level. Using an integrated approach involving Tandem Mass Tag labeling and liquid chromatography–mass spectrometry analysis, proteomes of papaya fruit at different ripening stages were investigated. A total of 3220 proteins were identified, of which 2818 were quantified. The differential accumulated proteins (DAPs) exhibited various biological functions and diverse subcellular localizations. Among the DEPs, most of the pathogen defense-related proteins were down-regulated, suggesting that disease resistance decreased during the ripening process. The Kyoto Encyclopedia of Genes and Genomes enrichment analysis revealed that various metabolic pathways were significantly altered, particularly in flavonoid and fatty acid metabolisms. The up-regulation of several flavonoid biosynthesis-related proteins may provide more raw materials for pigment biosynthesis, accelerating the color variation of papaya fruit. Thus, variations in the fatty acid metabolism-related enzymes were investigated. For example, a lipoxygenase, which catalyzes the conversion of ACC to ethylene, was significantly induced, suggesting a cross-talk between the lipoxygenase-mediated fatty acid metabolism and the hormone-controlled fruit ripening in papaya. Furthermore, the contents of several important fatty acids were determined, and increased unsaturated fatty acids may be associated with papaya fruit volatile formation. Our data may give an intrinsic explanation of the variations in metabolism during the ripening process of papaya fruit and serve as a comprehensive resource for investigating the regulation mechanism involved.
HostingRepository	PRIDE
AnnounceDate	2019-11-12
AnnouncementXML	Submission_2019-11-12_10:24:15.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Chenyu Lu
SpeciesList	scientific name: Carica papaya; NCBI TaxID: 3649;
ModificationList	No PTMs are included in the dataset
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2018-02-06 08:09:33	ID requested
⏵ 1	2019-11-12 10:24:16	announced

Jiang B, Ou S, Xu L, Mai W, Ye M, Gu H, Zhang T, Yuan C, Shen C, Wang J, Liu K, Comparative proteomic analysis provides novel insights into the regulation mechanism underlying papaya (Carica papaya L.) exocarp during fruit ripening process. BMC Plant Biol, 19(1):238(2019) [pubmed]

curator keyword: Biological

submitter keyword: Papaya, ripening, differential expressed protein, metabolism, fatty acid

Chenjia Shen
contact affiliation	College of Life and Environmental Science, Hangzhou Normal University, Hangzhou 310036, China; Zhejiang Provincial Key Laboratory for Genetic Improvement and Quality Control of Medicinal Plants, Hangzhou Normal University, Hangzhou 310036, China
contact email	shencj@hznu.edu.cn
lab head
Chenyu Lu
contact affiliation	Zhejiang University
contact email	lcyyz@hotmail.com
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD008871
     1. Label: PRIDE project
     2. Name: Comparative proteomic analysis provides novel insights into the regulation mechanism underlying the fruit ripening process in papaya (Carica papaya L.)