PXD008858

PXD008858 is an original dataset announced via ProteomeXchange.

Title	Argentilactone molecular targets in Paracoccidioides brasiliensis identified by chemoproteomics
Description	Paracoccidioidomycosis (PCM) is the cause of several deaths from systemic mycoses. The etiological agents of PCM belong to the Paracoccidioides genus, which is restricted to Latin America. The infection is acquired through inhalation of conidia that primarily lodges in the lungs and may disseminate to other organs/tissues. Treatment of PCM is commonly achieved via the administration of antifungals such as amphotericin B, co-trimoxazole, and itraconazole. The antifungal toxicity and side effects, in addition to the long treatment time, have driven research for new bioactive compounds. Argentilactone, a compound isolated from the Brazilian savanna plant Hyptis ovaliofolia, has been suggested to be a potent antifungal, inhibiting the dimorphism of P. brasiliensis and enzymatic activity of isocitrate lyase, a key enzyme of the glyoxylate cycle. Furthermore, argentilactone has no cytotoxicity and genotoxicity in fibroblast cells at concentrations that inhibit fungal growth. This work was developed due to the importance of elucidating the putative mode of action of argentilactone. The chemoproteomics approach, by affinity chromatography, is the methodology used to explore the interactions between P. brasiliensis proteins and argentilactone. A total of 109 proteins was identified and classified functionally, with those related to amino acid metabolism, energy, and detoxification being the most representative. The interactome of argentilactone binding proteins was predicted. Argentilactone inhibited the enzymatic activity of malate dehydrogenase, citrate synthase, and pyruvate dehydrogenase. Furthermore, argentilactone induced the production of reactive oxygen species. In addition, our data were compared to previously obtained proteomics and transcriptional data. Altogether, our results reveal argentilactone as a promising antifungal.
HostingRepository	PRIDE
AnnounceDate	2018-02-14
AnnouncementXML	Submission_2019-02-26_07:57:03.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Lívia Do Carmo Silva
SpeciesList	scientific name: fungal sp. 5OL6-6PL1; NCBI TaxID: 439733;
ModificationList	No PTMs are included in the dataset
Instrument	nanoACQUITY UPLC

Revision	Datetime	Status	ChangeLog Entry
0	2018-02-05 07:41:54	ID requested
1	2018-02-07 15:15:27	announced
2	2018-02-14 02:02:04	announced	Updated project metadata.
⏵ 3	2019-02-26 07:57:04	announced	Updated project metadata.

Silva LDC, Tauhata SBF, Baeza LC, de Oliveira CMA, Kato L, Borges CL, de Almeida Soares CM, Pereira M, Argentilactone Molecular Targets in Paracoccidioides brasiliensis Identified by Chemoproteomics. Antimicrob Agents Chemother, 62(11):(2018) [pubmed]

curator keyword: Biological

submitter keyword: Paracoccidioides, antifungal, targets, argentilactone, chemoproteomic

Lívia do Carmo Silva
contact affiliation	Universidade Federal de Goiás
contact email	livia.lbm.ufg@gmail.com
lab head
Lívia Do Carmo Silva
contact affiliation	Universidade Federal de Goiás
contact email	livia.lbm.ufg@gmail.com
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD008858
     1. Label: PRIDE project
     2. Name: Argentilactone molecular targets in Paracoccidioides brasiliensis identified by chemoproteomics