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PXD008857

PXD008857 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitlePSI supercomplex copurification using PSI His-tag
DescriptionChlamydomonas reinhardtii PSI-Cyt b6f complexes from sucrose density gradient fractions were identified by western blot and SMF spectroscopy, but showed high heterogeneity, as evident from the multiple bands present in the Coomassie stained SDS gel and original EM micrographs. Therefore potential PSI-Cyt b6f supercomplexes were enriched by a subsequent PsaA HisTag purification step. Only this additional enrichment enabled the identification of novel PSI supercomplexes, although the sample remained far from being homogeneous. This dataset describes the quantitative mass spectrometric analysis of the enriched PSI supercomplex fraction in comparison to unpurified samples using metabolic 15N labeling.
HostingRepositoryPRIDE
AnnounceDate2024-10-22
AnnouncementXMLSubmission_2024-10-22_03:59:31.503.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterStefan Schulze
SpeciesList scientific name: Chlamydomonas reinhardtii; NCBI TaxID: 3055;
ModificationListiodoacetamide derivatized residue
InstrumentQ Exactive
Dataset History
RevisionDatetimeStatusChangeLog Entry
02018-02-05 07:27:20ID requested
12018-10-03 08:48:08announced
22024-10-22 03:59:34announced2024-10-22: Updated project metadata.
Publication List
10.1073/pnas.1809973115;
Steinbeck J, Ross IL, Rothnagel R, G, รค, belein P, Schulze S, Giles N, Ali R, Drysdale R, Sierecki E, Gambin Y, Stahlberg H, Takahashi Y, Hippler M, Hankamer B, promoting cyclic electron flow under anaerobic conditions. Proc Natl Acad Sci U S A, 115(41):10517-10522(2018) [pubmed]
Keyword List
curator keyword: Biological
submitter keyword: His-tag,PSI, supercomplex, Chlamydomonas reinhardtii
Contact List
Michael Hippler
contact affiliationInstitute of Plant Biology and Biotechnology, University of Muenster
contact emailmhippler@uni-muenster.de
lab head
Stefan Schulze
contact affiliationUniversity of Pennsylvania
contact emailsschulze@sas.upenn.edu
dataset submitter
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Dataset FTP location
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PRIDE project URI
Repository Record List
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