PXD008855

PXD008855 is an original dataset announced via ProteomeXchange.

Title	Proteoglycan sequencing
Description	Proteoglycans are distributed in all animal tissues and play critical, multifaceted, physiological roles. Expressed in a spatially- and temporally-regulated manner, these molecules regulate interactions among growth factors and cell surface receptors and play key roles in basement membranes and other extracellular matrices. Due to the high degree of glycosylation by glycosaminoglycan (GAG), N-glycan and mucin-type O-glycan classes, the peptide sequence coverage of complex proteoglycans is revealed poorly by standard mass spectrometry-based proteomics methods. As a result, there is little information concerning how proteoglycan site specific glycosylation changes during normal and pathological processes. Here, we developed a workflow to improve sequence coverage and identification of glycosylated peptides in proteoglycans. We applied this workflow to the small leucine-rich proteoglycan decorin and the hyalectan proteoglycans; neurocan, brevican, and aggrecan.
HostingRepository	PRIDE
AnnounceDate	2018-05-22
AnnouncementXML	Submission_2018-05-22_04:15:15.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD008855
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Joseph Zaia
SpeciesList	scientific name: Bos taurus (Bovine); NCBI TaxID: 9913; scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	Phospho; Carbamidomethyl
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2018-02-05 06:56:03	ID requested
⏵ 1	2018-05-22 04:15:17	announced

Klein JA, Meng L, Zaia J, Deep Sequencing of Complex Proteoglycans: A Novel Strategy for High Coverage and Site-specific Identification of Glycosaminoglycan-linked Peptides. Mol Cell Proteomics, 17(8):1578-1590(2018) [pubmed]

curator keyword: Biological

submitter keyword: decorin, neurocan, brevican, aggrecan, LC-MS

Joseph Zaia
contact affiliation	Boston University, Dept. of Biochemistry
contact email	jzaia@bui.edu
lab head
Joseph Zaia
contact affiliation	Boston University
contact email	jzaia@bu.edu
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2018/05/PXD008855

PRIDE project URI

• PRIDE
  1. PXD008855
     1. Label: PRIDE project
     2. Name: Proteoglycan sequencing