PXD008853

PXD008853 is an original dataset announced via ProteomeXchange.

Title	On the characterisation of the porcine gland-specific salivary proteome using MALDI-TOF/TOF MS and Q-Exactive orbitrap MS/MS
Description	To expand the knowledge on the porcine salivary proteome, secretions from the three major salivary glands were collected from anaesthetised piglets. Pilocarpine and isoproterenol were simultaneously injected intraperitoneally to increase the volume and protein concentration of the saliva, respectively. The protein composition and relative protein-specific abundance of saliva secreted by the parotid gland and by the mandibular and monostomatic sublingual gland, were determined using iTRAQ. When combining two detection methods, MALDI-TOF/TOF MS and Q-Exactive orbitrap MS/MS, a total of 122 porcine salivary proteins and 6 mammalian proteins with a predicted porcine homolog were identified. Only a quantitative and not a qualitative difference was observed between both ductal secretions. The 128 proteins were detected in both secretions, however, at different levels. Twenty-four proteins (20 porcine, 4 mammalian with a predicted porcine homolog) were predominantly secreted by the parotid gland, such as carbonic anhydrase VI and alpha-amylase. Twenty-nine proteins (all porcine) were predominantly secreted by the mandibular and sublingual glands, for example salivary lipocalin and submaxillary apomucin protein.
HostingRepository	PRIDE
AnnounceDate	2019-02-15
AnnouncementXML	Submission_2019-02-15_10:16:16.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Sara Prims
SpeciesList	scientific name: Sus scrofa domesticus (domestic pig); NCBI TaxID: 9825;
ModificationList	methylthiolated residue; iTRAQ8plex-116 reporter+balance reagent acylated residue; monohydroxylated residue
Instrument	Q Exactive; 4800 Proteomics Analyzer

Revision	Datetime	Status	ChangeLog Entry
0	2018-02-05 06:44:22	ID requested
⏵ 1	2019-02-15 10:16:17	announced

Prims S, Van Raemdonck G, Vanden Hole C, Van Cruchten S, Van Ginneken C, Van Ostade X, Casteleyn C, On the characterisation of the porcine gland-specific salivary proteome. J Proteomics, 196():92-105(2019) [pubmed]

curator keyword: Biological

submitter keyword: Pig, salivary proteome, gland-specific saliva, iTRAQ, LC-MS

Chris Van Ginneken
contact affiliation	Applied Veterinary Morphology, Department of Veterinary Sciences, University of Antwerp, Belgium
contact email	chris.vanginneken@uantwerpen.be
lab head
Sara Prims
contact affiliation	UA
contact email	sara.prims@gmail.com
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2019/02/PXD008853

PRIDE project URI

• PRIDE
  1. PXD008853
     1. Label: PRIDE project
     2. Name: On the characterisation of the porcine gland-specific salivary proteome using MALDI-TOF/TOF MS and Q-Exactive orbitrap MS/MS